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Literature summary for 3.2.1.132 extracted from

  • Zitouni, M.; Fortin, M.; Scheerle, R.K.; Letzel, T.; Matteau, D.; Rodrigue, S.; Brzezinski, R.
    Biochemical and molecular characterization of a thermostable chitosanase produced by the strain Paenibacillus sp. 1794 newly isolated from compost (2013), Appl. Microbiol. Biotechnol., 97, 5801-5813.
    View publication on PubMed
Search for more literature for 3.2.1.132

Inhibitors

Inhibitors Comment Organism Structure
chitosan substrate inhibition with Ki of 01.66 mg/ml Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Paenibacillus sp.
41800
-
 x * 41800, calculated from amino acid sequence Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Paenibacillus sp.
-
 ?
-
 ?
chitosan + H2O Paenibacillus sp. 1794
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. J7G4R4
-
-
Paenibacillus sp. 1794 J7G4R4
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and hydroxyapatite column chromatography Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O 58% activity compared to chitosan Paenibacillus sp. ?
-
 ?
carboxymethylcellulose + H2O 58% activity compared to chitosan Paenibacillus sp. 1794 ?
-
 ?
chitosan + H2O
-
 Paenibacillus sp. ?
-
 ?
chitosan + H2O
-
 Paenibacillus sp. 1794 ?
-
 ?
methylcellulose + H2O 14% activity compared to chitosan Paenibacillus sp. ?
-
 ?
methylcellulose + H2O 14% activity compared to chitosan Paenibacillus sp. 1794 ?
-
 ?
additional information does not hydrolyze microcrystalline cellulose, chitin, xylan, laminarin, pustulan, pachyman, mannan, or beta-1,3-1,4-glucan Paenibacillus sp. ?
-
 ?
additional information does not hydrolyze microcrystalline cellulose, chitin, xylan, laminarin, pustulan, pachyman, mannan, or beta-1,3-1,4-glucan Paenibacillus sp. 1794 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Paenibacillus sp.
? x * 41800, calculated from amino acid sequence Paenibacillus sp.

Synonyms

Synonyms Comment Organism
Csn
-
 Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 85 the half-life of the enzyme at 70°C in the presence of chitosan substrate is more than 20 h. Maximal activity is observed at 80°C for 10 min and at 85°C for 5 and 3 min incubation times Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
126.5
-
 chitosan at pH 4.8 and 37°C Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7 4.8
-
 Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.8 6.5 the enzyme keeps at least 80% of activity between pH 3.8 and 6.5 Paenibacillus sp.