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Literature summary for 3.2.1.132 extracted from
- Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239 (2010), Microb. Cell Fact., 9, 5.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the enzyme is expressed recombinantly as a periplasmic enzyme in Escherichia coli strain BMS172 | Janthinobacterium sp. 4239 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 33000, native enzyme, SDS-PAGE, x * 31000, recombinant enzyme, SDS-PAGE | Janthinobacterium sp. 4239 |
| 33000 | - |
x * 33000, native enzyme, SDS-PAGE, x * 31000, recombinant enzyme, SDS-PAGE | Janthinobacterium sp. 4239 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Janthinobacterium sp. 4239 | chitosanases hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Janthinobacterium sp. 4239 | - |
isolated from a fresh water lake close to Kangerlussuaq in West Greenland | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | hydrolysis of chitosan hexamer, chitosan substrates are 55-80% deacetylated | Janthinobacterium sp. 4239 | D-glucosamine + ? | dimers and trimers | ? | |
| additional information | chitosanases hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues | Janthinobacterium sp. 4239 | ? | - |
? | |
| additional information | the enzyme is specific for chitosan as substrate, no activity with colloidal chitin, insoluble cellulose, CM cellulose, beta-1,3-glucan, xylan, and arabinoxylan | Janthinobacterium sp. 4239 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, native enzyme, SDS-PAGE, x * 31000, recombinant enzyme, SDS-PAGE | Janthinobacterium sp. 4239 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the chitosanase is related to the glycosyl hydrolase family 46 chitosanases | Janthinobacterium sp. 4239 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Janthinobacterium sp. 4239 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | 20%, 30%, and 70% of the maximum activity at 60°C, 10°C, and 30°C, respectively, maximal activity at 45°C | Janthinobacterium sp. 4239 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the recombinant chitosanase is stable at temperatures up to 50°C above which the enzyme activity decreases rapidly | Janthinobacterium sp. 4239 |
| 70 | - |
the recombinant chitosanase shows hardly activity after 30 min | Janthinobacterium sp. 4239 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Janthinobacterium sp. 4239 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | the recombinant enzyme is inactive above pH 8.0 and shows 90% of maximal activity at pH 4.0 | Janthinobacterium sp. 4239 |
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