Literature summary for 3.2.1.132 extracted from

Ngo, K.X.; Umakoshi, H.; Shimanouchi, T.; Sugaya, H.; Kuboi, R.
Chitosanase displayed on liposome can increase its activity and stability (2010), J. Biotechnol., 146, 105-113.

Search for more literature for 3.2.1.132

Protein Variants

Protein Variants	Comment	Organism
additional information	immobilization of chitosanase onto liposome for construction of a biocatalyst, the immobilized enzyme on liposomes shows increased activity and stability to pH and temperature, overview	Streptomyces griseus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics, overview	Streptomyces griseus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Streptomyces griseus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	x * 39000, enzyme with signal peptide, SDS-PAGE	Streptomyces griseus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Streptomyces griseus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.38	1.53	activity of enzyme immobilized on liposomes	Streptomyces griseus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycol chitosan + H2O	hydrolysis of glycol chitosan with release of glucosamine	Streptomyces griseus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 39000, enzyme with signal peptide, SDS-PAGE	Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	assay at	Streptomyces griseus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.6	-	assay at	Streptomyces griseus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)