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Literature summary for 3.2.1.132 extracted from

  • Lin, Y.; Hsiao, Y.; Chiang, B.
    Production of high degree polymerized chitooligosaccharides in a membrane reactor using purified chitosanase from Bacillus cereus (2009), Food Res. Int., 42, 1355-1361.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Bacillus cereus
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-
-
Bacillus cereus NTU-FC-4
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-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 281fold by three steps of anion exchange chromatography Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan hexasaccharide + H2O
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Bacillus cereus chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
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?
chitosan hexasaccharide + H2O
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Bacillus cereus NTU-FC-4 chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
-
?
chitosan pentasaccharide + H2O hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3 Bacillus cereus chitosan disaccharide + chitosan trisaccharide
-
?
chitosan pentasaccharide + H2O hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3 Bacillus cereus NTU-FC-4 chitosan disaccharide + chitosan trisaccharide
-
?
additional information hydrolysis profiles, overview Bacillus cereus ?
-
?
additional information hydrolysis profiles, overview Bacillus cereus NTU-FC-4 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Bacillus cereus