Cloned (Comment) | Organism |
---|---|
wild-type and mutants subcloned into vector pFD666 and expressed in Streptomyces lividans TK-24 | Streptomyces sp. N174 |
Protein Variants | Comment | Organism |
---|---|---|
D201A | time-course is almost identical to that obtained by the wild-type | Streptomyces sp. N174 |
D40G | reduces activity to 2% | Streptomyces sp. N174 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. N174 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by a two-step procedure involving ion exchange chromatography followed by gel filtration, to purity | Streptomyces sp. N174 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O | - |
Streptomyces sp. N174 | GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + D-glucosamine | - |
? | |
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O | - |
Streptomyces sp. N174 | GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN | - |
? | |
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O | - |
Streptomyces sp. N174 | GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN | - |
? | |
hexa-N-acetylchitohexaose + H2O | - |
Streptomyces sp. N174 | ? | - |
? | |
additional information | does not hydrolyze GlcNbeta(1-4)GlcN or GlcNbeta(1-4)GlcNbeta(1-4)GlcN | Streptomyces sp. N174 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endochitosanase | - |
Streptomyces sp. N174 |