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Literature summary for 3.2.1.132 extracted from

  • Wang, J.; Zhou, W.; Yuan, H.; Wang, Y.
    Characterization of a novel fungal chitosanase Csn2 from Gongronella sp. JG (2008), Carbohydr. Res., 343, 2583-2588.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genomic sequence of Csn2 cloned from the DNA library by PCR Gongronella sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibits Csn2 activity at 10 mM Gongronella sp.
EDTA
-
Gongronella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Gongronella sp.
Cu2+ stimulates Csn2 at 1 mM Gongronella sp.
Mn2+ activates Gongronella sp.
Sr2+ activates Gongronella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Gongronella sp.

Organism

Organism UniProt Comment Textmining
Gongronella sp. B0LV85
-
-
Gongronella sp. JG-2005 B0LV85
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Gongronella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitosan + H2O 85% deacetylated, Csn2 displays stronger hydrolysis capability against colloidal chitosan than soluble chitosan Gongronella sp. chitobiose + chitotriose + chitotetraose
-
?
colloidal chitosan + H2O 85% deacetylated, Csn2 displays stronger hydrolysis capability against colloidal chitosan than soluble chitosan Gongronella sp. JG-2005 chitobiose + chitotriose + chitotetraose
-
?
glycol chitosan + H2O weak activity against glycol chitosan, Csn2 displays 4% of the activity for colloidal chitosan Gongronella sp. ?
-
?
glycol chitosan + H2O weak activity against glycol chitosan, Csn2 displays 4% of the activity for colloidal chitosan Gongronella sp. JG-2005 ?
-
?
additional information does not hydrolyze colloidal chitin and carboxylmethyl cellulose Gongronella sp. ?
-
?
additional information does not hydrolyze colloidal chitin and carboxylmethyl cellulose Gongronella sp. JG-2005 ?
-
?
soluble chitosan + H2O 85% deacetylated, Csn2 displays 14% of the activity for colloidal chitosan Gongronella sp. ?
-
?
soluble chitosan + H2O 85% deacetylated, Csn2 displays 14% of the activity for colloidal chitosan Gongronella sp. JG-2005 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Gongronella sp.

Synonyms

Synonyms Comment Organism
Csn2
-
Gongronella sp.
GH-75 chitosanase
-
Gongronella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60 optimal reaction temperature is between 55°C and 60°C Gongronella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 55 half-lives of Csn2 at 50°C and 55°C are 30 min and 11 min, respectively Gongronella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Gongronella sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.2 5.8
-
Gongronella sp.