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Literature summary for 3.2.1.132 extracted from

  • Wang, S.L.; Peng, J.H.; Liang, T.W.; Liu, K.C.
    Purification and characterization of a chitosanase from Serratia marcescens TKU011 (2008), Carbohydr. Res., 343, 1316-1323.
    View publication on PubMed
Search for more literature for 3.2.1.132

Activating Compound

Activating Compound Comment Organism Structure
additional information chitosanase is activated after keeping at 4°C for 10 days, in the presence of 25% (v/v) toluene, acetonitrile, and butanol Serratia marcescens

Application

Application Comment Organism
food industry usage of strain TKU011 on the microbial reclamation of food processing wastes such as shrimp shell wastes for the production of chitosanase Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM inhibits by 92% at 37°C for 30 min Serratia marcescens
EDTA 5 mM completely inhibits at 37°C for 30 min Serratia marcescens
Fe2+ 5 mM completely inhibits at 37°C for 30 min Serratia marcescens
Mn2+ 5 mM completely inhibits at 37°C for 30 min Serratia marcescens
additional information 5 mM Ba2+, Mg2+ and Ca2+ have little effect on activity at 37°C for 30 min. After keeping at 25°C for 10 days, the chitosanase is inactivated in the presence of 25% (v/v) ethanol and ethyl ether Serratia marcescens
PMSF 5 mM inhibits by 73% at 37°C for 30 min Serratia marcescens
Zn2+ 5 mM inhibits by 49% at 37°C for 30 min Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 gel filtration Serratia marcescens
18780
-
 sequence analysis Serratia marcescens
21000
-
 1 * 21000, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-
Serratia marcescens TKU011
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by a combination of ion-exchange and gel-filtration, 50fold with an overall activity yield of 24% Serratia marcescens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 culture supernatant Serratia marcescens
7
-
 50fold purified enzyme Serratia marcescens

Storage Stability

Storage Stability Organism
25°C, the chitosanase is inactivated in the presence of 25% (v/v) ethanol and ethyl ether, 10 days Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 60-95% degree of deacetylation Serratia marcescens ?
-
 ?
chitosan + H2O activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 60-95% degree of deacetylation Serratia marcescens TKU011 ?
-
 ?
additional information no activity against colloidal chitin, chitin (a-type) and chitin (b-type). The enzyme has antioxidant activity Serratia marcescens ?
-
 ?
additional information no activity against colloidal chitin, chitin (a-type) and chitin (b-type). The enzyme has antioxidant activity Serratia marcescens TKU011 ?
-
 ?
shrimp-shell powder + H2O sole carbon/nitrogen source Serratia marcescens ?
-
 ?
shrimp-shell powder + H2O sole carbon/nitrogen source Serratia marcescens TKU011 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 21000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 maintains more than 90% of its initial activity between 25 and 50°C, but is rapidly inactivated retaining only 60% of its initial activity at 60°C, and is completely inactivated at greater than 70°C Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Serratia marcescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8
-
 Serratia marcescens

pI Value

Organism Comment pI Value Maximum pI Value
Serratia marcescens sequence analysis
-
 7.11