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Literature summary for 3.2.1.132 extracted from

  • Zhou, W.; Yuan, H.; Wang, J.; Yao, J.
    Production, purification and characterization of chitosanase produced by Gongronella sp. JG (2008), Lett. Appl. Microbiol., 46, 49-54.
    View publication on PubMed

Application

Application Comment Organism
synthesis optimization of production conditions. In the optimized medium, strain JG produces 0.8 mmol per min and l of enzymic activity in 72 h Gongronella sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 3% residaul activity Gongronella sp.
EDTA 1 mM, 78% residual activity Gongronella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 100% stimulation at 1 and 10 mM Gongronella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
x * 90000, SDS-PAGE Gongronella sp.

Organism

Organism UniProt Comment Textmining
Gongronella sp.
-
strain JG
-
Gongronella sp. JG-2005
-
strain JG
-

Purification (Commentary)

Purification (Comment) Organism
-
Gongronella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82
-
pH 4.8, 50°C Gongronella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O substrate with 85% deacetylation Gongronella sp. chitosan oligosaccharides
-
?
chitosan + H2O substrate with 85% deacetylation Gongronella sp. JG-2005 chitosan oligosaccharides
-
?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Gongronella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Gongronella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life 65 min Gongronella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6 4.8
-
Gongronella sp.

pI Value

Organism Comment pI Value Maximum pI Value
Gongronella sp. isoelectric focusing
-
5.8