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Literature summary for 3.2.1.132 extracted from

  • Sun, Y.; Liu, W.; Han, B.; Zhang, J.; Liu, B.
    Purification and characterization of two types of chitosanase from a Microbacterium sp. (2006), Biotechnol. Lett., 28, 1393-1399.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
chitosanase ChiN Microbacterium sp.
81000
-
chitosanase ChiX Microbacterium sp.

Organism

Organism UniProt Comment Textmining
Microbacterium sp.
-
chitosanase ChiN
-
Microbacterium sp.
-
chitosanase ChiX
-
Microbacterium sp. QU01
-
chitosanase ChiN
-
Microbacterium sp. QU01
-
chitosanase ChiX
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaose + H2O ChiN hydrolyzes oligomers larger than chitopentaose Microbacterium sp. ?
-
?
chitohexaose + H2O ChiN hydrolyzes oligomers larger than chitopentaose Microbacterium sp. QU01 ?
-
?
chitosan + H2O activity increases with the degree of deacetylation of chitosan Microbacterium sp. ?
-
?
chitosan + H2O activity increases with the degree of deacetylation of chitosan Microbacterium sp. QU01 ?
-
?
chitosan + H2O activity increases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 62-100% degree of deacetylation. Hydrolysis of chitosan with 99% degree of deacetylation by ChiN releases chitobiose, chitotriose and chitotetraose Microbacterium sp. chitobiose + chitotriose + chitotetraose
-
?
chitosan + H2O activity increases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 62-100% degree of deacetylation. Hydrolysis of chitosan with 99% degree of deacetylation by ChiN releases chitobiose, chitotriose and chitotetraose Microbacterium sp. QU01 chitobiose + chitotriose + chitotetraose
-
?

Synonyms

Synonyms Comment Organism
ChiN
-
Microbacterium sp.
ChiX
-
Microbacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
chitosanase ChiN Microbacterium sp.
60
-
chitosanase ChiX Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
chitosanase ChiN Microbacterium sp.
6.6
-
chitosanase ChiX Microbacterium sp.