Literature summary for 3.2.1.132 extracted from

Honda, Y.; Kirihata, M.; Fukamizo, T.; Kaneko, S.; Tokuyasu, K.; Brzezinski, R.
Chitosanase-catalyzed hydrolysis of 4-methylumbelliferyl beta-chitotrioside (1999), J. Biochem., 126, 470-474.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	4-methylumbelliferyl beta-chitotrioside	40°C, pH 5.5	Streptomyces sp.
2.9	-	chitohexaose	40°C, pH 5.5	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	-	N174	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl beta-chitotrioside + H2O	-	Streptomyces sp.	4-methylumbelliferone + chitotriose + chitobiose + 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000081	-	4-methylumbelliferyl beta-chitotrioside	40°C, pH 5.5	Streptomyces sp.
107	-	chitohexaose	40°C, pH 5.5	Streptomyces sp.

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Release 2023.1 (January 2023)