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Literature summary for 3.2.1.132 extracted from

  • Zhang, X.Y.; Dai, A.L.; Zhang, X.K.; Kuroiwa, K.; Kodaira, R.; Shimosaka, M.; Okazaki, M.
    Purification and characterization of chitosanase and exo-beta-D-glucosaminidase from a Koji mold, Aspergillus oryzae IAM2660 (2000), Biosci. Biotechnol. Biochem., 64, 1896-1902.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus oryzae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE, enzyme I Aspergillus oryzae
135000
-
x * 135000, SDS-PAGE, enzyme II Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Aspergillus oryzae
-
?
-
?
chitosan + H2O Aspergillus oryzae IAM2660
-
?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
IAM2660
-
Aspergillus oryzae IAM2660
-
IAM2660
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.2
-
enzyme I after after Sephadex G-100 chromatography Aspergillus oryzae
38.8
-
enzyme II after after Sephadex G-100 chromatography Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
Aspergillus oryzae ?
-
?
chitosan + H2O
-
Aspergillus oryzae IAM2660 ?
-
?
chitosan + H2O enzyme I prefers chitosan with 30% acetylation, but enzyme II mostly effects chitosan without acetylation Aspergillus oryzae additional information
-
?
chitosan + H2O enzyme I prefers chitosan with 30% acetylation, but enzyme II mostly effects chitosan without acetylation Aspergillus oryzae IAM2660 additional information
-
?

Subunits

Subunits Comment Organism
? x * 135000, SDS-PAGE, enzyme II Aspergillus oryzae
? x * 40000, SDS-PAGE, enzyme I Aspergillus oryzae