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Literature summary for 3.2.1.132 extracted from

  • Yoshihara, K.; Hosokawa, J.; Kubo, T.; Nishiyama, M.; Koba, Y.
    Purification and properties of a chitosanase from Pseudomonas sp. H-14 (1992), Biosci. Biotechnol. Biochem., 56, 972-973.
    View publication on PubMed
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Pseudomonas sp.
-
 ?
-
 ?
chitosan + H2O Pseudomonas sp. H-14
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 H-14
-
Pseudomonas sp. H-14
-
 H-14
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51.8
-
 after TSKgel G2000SWXL chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Pseudomonas sp. ?
-
 ?
chitosan + H2O
-
 Pseudomonas sp. H-14 ?
-
 ?
chitosan + H2O high substrate specificity for highly deacetylated chitosan Pseudomonas sp. additional information mixture of dimer and trimer of glucosamine ?
chitosan + H2O high substrate specificity for highly deacetylated chitosan Pseudomonas sp. H-14 additional information mixture of dimer and trimer of glucosamine ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Pseudomonas sp.