Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.132 extracted from

  • Okajima, S.; Ando, A.; Shinoyama, H.; Fujii, T.
    Purification and characterization of an extracellular chitosanase produced by Amycolatopsis sp. CsO-2 (1994), J. Ferment. Bioeng., 77, 617-620.
No PubMed abstract available
Search for more literature for 3.2.1.132

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Amycolatopsis sp.
N-bromosuccinimide
-
 Amycolatopsis sp.
p-chloromercuribenzoate
-
 Amycolatopsis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Amycolatopsis sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Amycolatopsis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Amycolatopsis sp.
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis sp.
-
 CsO-2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Amycolatopsis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Amycolatopsis sp. ?
-
 ?
chitosan + H2O most susceptible substrate is: 100% deacetylated chitosan Amycolatopsis sp. additional information
-
 ?
chitosan + H2O deacetylation degree from 70% to 100% Amycolatopsis sp. additional information
-
 ?
additional information no activity with chitin (i.e. 100% acetylated chitosan) Amycolatopsis sp. ?
-
 ?
additional information no activity with glucosamine dimer and trimer Amycolatopsis sp. ?
-
 ?
additional information degrades glucosamine tetramer to dimer and pentamer and to dimer and trimer Amycolatopsis sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Amycolatopsis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 pH 5.3 Amycolatopsis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 stable Amycolatopsis sp.
50
-
 60 min, stable Amycolatopsis sp.
60
-
 60 min, 40% loss of activity Amycolatopsis sp.
70
-
 60 min, 70% loss of activity Amycolatopsis sp.
80
-
 60 min, 80% loss of activity Amycolatopsis sp.
100
-
 10 min, 50% loss of activity Amycolatopsis sp.