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Literature summary for 3.2.1.132 extracted from
- Chitosanase from Streptomyces griseus (1988), Methods Enzymol., 161, 505-510.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Streptomyces griseus |  |
| Cu2+ | - |
Streptomyces griseus | |
| Fe2+ | - |
Streptomyces griseus | |
| Hg2+ | - |
Streptomyces griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.18% carboxymethylcellulose | Streptomyces griseus | |
| additional information | - |
additional information | 0.21% glycol chitosan | Streptomyces griseus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10000 | - |
gel filtration | Streptomyces griseus |
| 35000 | - |
x * 35000, SDS-PAGE | Streptomyces griseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Streptomyces griseus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces griseus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.76 | - |
after second Sephadex G-75 chromatography | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Streptomyces griseus | ? | - |
? | |
| carboxymethylchitosan + H2O | - |
Streptomyces griseus | ? | - |
? | |
| chitosan + H2O | - |
Streptomyces griseus | ? | - |
? | |
| chitosan + H2O | hydrolysis of: soluble chitosan | Streptomyces griseus | additional information | - |
? | |
| chitosan + H2O | hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated | Streptomyces griseus | additional information | - |
? | |
| chitosan + H2O | hydrolysis of: glycol chitosan | Streptomyces griseus | additional information | - |
? | |
| additional information | no activity with glycol chitin | Streptomyces griseus | ? | - |
? | |
| additional information | no activity with colloidal chitin | Streptomyces griseus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Streptomyces griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 6.0-8.0, stable, unstable at elevated temperatures | Streptomyces griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
carboxymethylcellulose hydrolysis | Streptomyces griseus |
| 8 | - |
chitosanase activity | Streptomyces griseus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces griseus | - |
- |
9.7 |
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