Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 with an N-terminal His tag | Amphibacillus xylanus |
the enzyme is expressed in Escherichia coli BL21 (DE3) CodonPlus in fusion with an N-terminal His tag | Amphibacillus xylanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 94% loss of activity | Amphibacillus xylanus | |
AgNO3 | 1 mM, 94% loss of activity | Amphibacillus xylanus | |
Cu2+ | 1 mM, 49% loss of activity | Amphibacillus xylanus | |
CuSO4 | 1 mM, 49% loss of activity | Amphibacillus xylanus | |
additional information | addition of 1 mM Mg2+, Ca2+, Fe3+ or Zn2+ has no effect | Amphibacillus xylanus | |
Ni2+ | 1 mM, 12% loss of activity | Amphibacillus xylanus | |
NiCl2 | 1 mM, 12% loss of activity | Amphibacillus xylanus | |
Sr2+ | 1 mM, 17% loss of activity | Amphibacillus xylanus | |
SrCl2 | 1 mM, 17% loss of activity | Amphibacillus xylanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose | Km-value: 2.4 mg/ml, pH 7.0, 55°C | Amphibacillus xylanus | |
additional information | - |
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose | Km-value: 5.0 mg/ml, pH 7.0, 55°C | Amphibacillus xylanus | |
additional information | - |
arabinoglucuronoxylan | Km-value: 50 mg/ml, pH 7.0, 55°C | Amphibacillus xylanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 1 mM Mg2+, Ca2+, Fe3+ or Zn2+ has no effect on activity | Amphibacillus xylanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109960 | - |
calculated from sequence | Amphibacillus xylanus |
110000 | - |
SDS-PAGE | Amphibacillus xylanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amphibacillus xylanus | K0J4X5 | - |
- |
Amphibacillus xylanus ATCC 51415 | K0J4X5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Amphibacillus xylanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose + H2O | - |
Amphibacillus xylanus | 4-O-methyl-glucuronic acid + ? | - |
? | |
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose + H2O | - |
Amphibacillus xylanus ATCC 51415 | 4-O-methyl-glucuronic acid + ? | - |
? | |
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O | - |
Amphibacillus xylanus | 4-O-methyl-glucuronic acid + ? | - |
? | |
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O | - |
Amphibacillus xylanus ATCC 51415 | 4-O-methyl-glucuronic acid + ? | - |
? | |
arabinoglucuronoxylan + H2O | - |
Amphibacillus xylanus | 4-O-methyl-glucuronic acid + ? | - |
? | |
arabinoglucuronoxylan + H2O | - |
Amphibacillus xylanus ATCC 51415 | 4-O-methyl-glucuronic acid + ? | - |
? | |
beechwood xylan + H2O | 83% of the activity as compared to spruce arabinoglucuronoxylan | Amphibacillus xylanus | 4-O-methyl-D-glucuronic acid + ? | - |
? | |
beechwood xylan + H2O | 83% of the activity as compared to spruce arabinoglucuronoxylan | Amphibacillus xylanus ATCC 51415 | 4-O-methyl-D-glucuronic acid + ? | - |
? | |
glucuronoxylan + H2O | - |
Amphibacillus xylanus | 4-O-methyl-glucuronic acid + ? | - |
? | |
additional information | the enzyme is similarly active towards glucuronoxylan as well as comparatively complex xylans such as spruce arabinoglucurunoxylan | Amphibacillus xylanus | ? | - |
? | |
additional information | the enzyme is similarly active towards glucuronoxylan as well as comparatively complex xylans such as spruce arabinoglucurunoxylan | Amphibacillus xylanus ATCC 51415 | ? | - |
? | |
oat spelt xylan + H2O | 8.9% of the activity as compared to spruce arabinoglucuronoxylan | Amphibacillus xylanus | 4-O-methyl-D-glucuronic acid + ? | - |
? | |
spruce arabinoglucuronoxylan + H2O | - |
Amphibacillus xylanus | 4-O-methyl-D-glucuronic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, SDS-PAGE | Amphibacillus xylanus |
Synonyms | Comment | Organism |
---|---|---|
AxyAgu115A | - |
Amphibacillus xylanus |
GH115 alpha-glucuronidase | - |
Amphibacillus xylanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Amphibacillus xylanus |
55 | - |
assay at | Amphibacillus xylanus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half life (t1/2): 24 h | Amphibacillus xylanus |
40 | - |
half life: 24 h | Amphibacillus xylanus |
45 | - |
1 h, enzyme retains 100% activity. Half life: 10 h | Amphibacillus xylanus |
45 | - |
the enzyme retains 100% activity following preincubation for 1 h. Half life (t1/2): 10 h | Amphibacillus xylanus |
50 | - |
1 h, enzyme retains 75% of its initial activity. Half life: 90 min | Amphibacillus xylanus |
50 | - |
the enzyme retains 75% activity following preincubation for 1 h. Half life (t1/2): 90 min | Amphibacillus xylanus |
55 | - |
1 h, complete loss of activity. Half life: 7 min | Amphibacillus xylanus |
55 | - |
no activity is detected after incubation for 1 h at temperatures above 55°C. Half life (t1/2): 7 min | Amphibacillus xylanus |
60 | - |
half life (t1/2): 5 min | Amphibacillus xylanus |
60 | - |
half life: 5 min | Amphibacillus xylanus |
65 | - |
half life (t1/2): 2 min | Amphibacillus xylanus |
65 | - |
half life: 2 min | Amphibacillus xylanus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.2 | - |
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose | pH 7.0, 55°C | Amphibacillus xylanus | |
72.7 | - |
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose | pH 7.0, 55°C | Amphibacillus xylanus | |
487.3 | - |
arabinoglucuronoxylan | pH 7.0, 55°C | Amphibacillus xylanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Amphibacillus xylanus |
7 | - |
activity on beechwood xylan | Amphibacillus xylanus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | the enzyme retains over 80% relative activity between pH 5.0 to 8.5 | Amphibacillus xylanus |
5 | 10.5 | relative activity between pH 5.0 and pH 8.5 was above 80%, and was 35% of maximum at pH 10.5, activity on beechwood xylan | Amphibacillus xylanus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
the enzyme loses 30% and 80% relative residual activity after 24 h preincubation | Amphibacillus xylanus |
10 | - |
the enzyme loses 30% and 80% relative residual activity after 24 h preincubation | Amphibacillus xylanus |