Application | Comment | Organism |
---|---|---|
pharmacology | the RG product vomilenine is a direct intermediate in ajmaline biosynthesis and utilization of raucaffricine for the ajmaline biosynthestic pathway could be a crucial and rate-limiting step in the formation of the antiarrythmic drug ajmaline | Rauvolfia serpentina |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli TOP 10, sequencing | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Raucaffricine | pH 5, 28°C, recombinant RG | Rauvolfia serpentina | |
1.8 | - |
strictosidine | pH 5, 28°C, recombinant RG | Rauvolfia serpentina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60931 | - |
x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
61000 | - |
x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
raucaffricine + H2O | Rauvolfia serpentina | specifically involved in alkaloid biosynthesis, e.g. ajmaline | D-glucose + vomilenine | vomilene is a direct intermediate in ajmaline biosynthesis | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | Q9SPP9 | BENTH. ex KURZ | - |
Purification (Comment) | Organism |
---|---|
native and recombinant RG | Rauvolfia serpentina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Rauvolfia serpentina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroraucaffricine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
1-methyl-1,2-dihydroraucaffricine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
21-glucopyranosyl-hydroxysarpagan-17-al + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
21-glucopyranosyl-hydroxysarpagan-17-ol + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
5alpha-carboxystrictosidine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
raucaffricine + H2O | best substrate, highly specific enzyme | Rauvolfia serpentina | D-glucose + vomilenine | - |
? | |
raucaffricine + H2O | specifically involved in alkaloid biosynthesis, e.g. ajmaline | Rauvolfia serpentina | D-glucose + vomilenine | vomilene is a direct intermediate in ajmaline biosynthesis | ? | |
strictosidine + H2O | only recombinant RG, no substrate of native RG | Rauvolfia serpentina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
Synonyms | Comment | Organism |
---|---|---|
More | belongs to family1 of glycosyl hydrolases | Rauvolfia serpentina |
RG | - |
Rauvolfia serpentina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Rauvolfia serpentina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Rauvolfia serpentina |