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Literature summary for 3.2.1.123 extracted from
- Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system (2012), J. Lipid Res., 53, 2242-2251.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dimethylformamide | the activity is enhanced by addition of 5% (v/v) dimethylformamide | Rhodococcus equi |  |
| Dimethylsulfoxide | the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide | Rhodococcus equi | |
| tetrahydrofuran | the activity is enhanced by addition of 5% (v/v) tetrahydrofuran | Rhodococcus equi | |
| Triton X-100 | the activity is enhanced by addition of 0.1% (v/v) Triton X-100 | Rhodococcus equi | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Rhodococcus erythropolis JCM3201 cells | Rhodococcus equi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
ganglioside Gb3Cer | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
| 0.4 | - |
ganglioside GM1a | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
| 0.43 | - |
ganglioside GM1a | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
| 0.46 | - |
ganglioside Gb3Cer | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, isoform EGCase I, SDS-PAGE | Rhodococcus equi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oligoglycosylglucosyl-(1<->1)-ceramide + H2O | Rhodococcus equi | - |
ceramide + oligoglycosylglucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus equi | - |
formerly Rhodococcus sp. strain M-750 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni Sepharose 6 column chromatography | Rhodococcus equi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-fucosyl-(1->2)-ganglioside GM1a + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| Forssman glycolipid + H2O | isoform EGCase I exhibits 25.2% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| ganglioside Gb3Cer + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| ganglioside Gb4Cer + H2O | isoform EGCase I exhibits 33.9% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| ganglioside GD1a + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| ganglioside GD1b + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| ganglioside GD3 + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| ganglioside GM1a + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| ganglioside GM3 + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| glucosylceramide + H2O | isoform EGCase I exhibits 6.7% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| lactosylceramide + H2O | isoform EGCase I exhibits 29.9% activity with this substrate | Rhodococcus equi | ? | - |
? | |
| additional information | almost no hydrolysis is observed for isoform EGCase II with alpha-L-fucosyl-(1->2)-ganglioside GM1a, ganglioside Gb3Cer, and ganglioside Gb4Cer. Isoform EGCase I shows no activity with ganglioside GM4, trigalactosylceramide, sulfatide, and sphingomyelin | Rhodococcus equi | ? | - |
? | |
| additional information | for transglycosylation activity, isoform EGCase I prefers alkanols with a relatively long carbon chain such as pentanol and hexanol as an acceptor substrate | Rhodococcus equi | ? | - |
? | |
| oligoglycosylglucosyl-(1<->1)-ceramide + H2O | - |
Rhodococcus equi | ceramide + oligoglycosylglucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, isoform EGCase I, SDS-PAGE | Rhodococcus equi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EGCase I | isoform | Rhodococcus equi |
| EGCase II | isoform | Rhodococcus equi |
| endoglycoceramidase | - |
Rhodococcus equi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.069 | - |
ganglioside Gb3Cer | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
| 5.7 | - |
ganglioside GM1a | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
| 85 | - |
ganglioside Gb3Cer | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
| 120 | - |
ganglioside GM1a | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | isoform EGCase I | Rhodococcus equi |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
ganglioside Gb3Cer | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
| 13 | - |
ganglioside GM1a | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
| 190 | - |
ganglioside Gb3Cer | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
| 320 | - |
ganglioside GM1a | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
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