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Literature summary for 3.2.1.123 extracted from

  • Horibata, Y.; Sakaguchi, K.; Okino, N.; Iida, H.; Inagaki, M.; Fujisawa, T.; Hama, Y.; Ito, M.
    Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase (2004), J. Biol. Chem., 279, 33379-33389.
    View publication on PubMed
Search for more literature for 3.2.1.123

Activating Compound

Activating Compound Comment Organism Structure
Lubrol Px 0.4% (w/v) Hydra vulgaris
Triton X-100 0.2% (w/v) Hydra vulgaris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Chinese hamster ovary cells and Escherichia coli Hydra vulgaris

Inhibitors

Inhibitors Comment Organism Structure
taurodeoxycholate 0.1-1% (w/v) Hydra vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no significant effect on the activity at 5 mM Hydra vulgaris
Cu2+ no significant effect on the activity at 5 mM Hydra vulgaris
Mg2+ no significant effect on the activity at 5 mM Hydra vulgaris
Mn2+ no significant effect on the activity at 5 mM Hydra vulgaris
Zn2+ no significant effect on the activity at 5 mM Hydra vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52900
-
 after treatment with glycopeptidase F, monomer, SDS-PAGE Hydra vulgaris
56000
-
 monomer, SDS-PAGE Hydra vulgaris
59680
-
 calculated from amino acid sequence Hydra vulgaris

Organism

Organism UniProt Comment Textmining
Aiptasia sp.
-
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Cellana sp.
-
-
-
Cyanea nozakii Q9GV16
-
-
Entacmaea sp.
-
-
-
Hydra vulgaris
-
-
-
Hydra vulgaris 105
-
-
-
Marphysa sp.
-
-
-
Meretrix sp.
-
-
-
Ruditapes sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hydra vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
additional information endodermal layer, especially digestive cells Hydra vulgaris
-
pineal peduncle
-
 Hydra vulgaris
-
stomach
-
 Hydra vulgaris
-
tentacle
-
 Hydra vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000032
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Marphysa sp.
0.0000044
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Meretrix sp.
0.0000046
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Ruditapes sp.
0.0000058
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Bacillus sp. (in: Bacteria)
0.0000118
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Cellana sp.
0.0000747
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Cyanea nozakii
0.00021
-
 gastric cavity, 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Hydra vulgaris
0.00023
-
 peduncle regions, 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Hydra vulgaris
0.0002325
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Hydra vulgaris
0.0002346
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Entacmaea sp.
0.0002403
-
 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Aiptasia sp.
0.00025
-
 tentacles, 37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate Hydra vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris 105 ceramide + Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Bacillus sp. (in: Bacteria) ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Cyanea nozakii ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Aiptasia sp. ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Entacmaea sp. ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Cellana sp. ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Meretrix sp. ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Ruditapes sp. ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Marphysa sp. ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris 105 ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris 105 ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + Galbeta(1,4)Glc
-
 ?
Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris 105 ceramide + Galbeta(1,4)Glc
-
 ?
Galbeta(1,6)Galbeta(1,6)Galbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100, no reaction Hydra vulgaris ?
-
 ?
Galbeta(1,6)Galbeta(1,6)Galbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100, no reaction Hydra vulgaris 105 ?
-
 ?
Galbeta(1,6)Galbeta(1,6)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + Galbeta(1,6)Galbeta(1,6)Glc
-
 ?
GalNAcalpha(1,4)GalNAcbeta(1,4)GlcNAcbeta(1,3)Manbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + GalNAcalpha(1,4)GalNAcbeta(1,4)GlcNAcbeta(1,3)Manbeta(1,4)Glc
-
 ?
GalNAcbeta(1,3)Galalpha(1,4)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + GalNAcbeta(1,3)Galalpha(1,4)Galbeta(1,4)Glc
-
 ?
GlcA4Me-beta(1,4)[GalNAc-Me-alpha(1,3)]Fucalpha(1,4)GlcNAcbeta(1,2)Manalpha(1,3)[Xylbeta(1,2)]Manbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + GlcA4Me-beta(1,4)[GalNAc-Me-alpha(1,3)]Fucalpha(1,4)GlcNAcbeta(1,2)Manalpha(1,3)[Xylbeta(1,2)]Manbeta(1,4)Glc
-
 ?
NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
-
 ?
NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100, most favoured substrate Hydra vulgaris ceramide + NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha-2,3]Gal-beta(1,4)Glc
-
 ?
NeuAcalpha(2,3)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O 37°C, pH 3.0, 0.2% (w/v) Triton X-100 Hydra vulgaris ceramide + NeuAcalpha(2,3)Galbeta(1,4)Glc
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Hydra vulgaris

Synonyms

Synonyms Comment Organism
EGCase
-
 Bacillus sp. (in: Bacteria)
EGCase
-
 Cyanea nozakii
EGCase
-
 Hydra vulgaris
EGCase
-
 Aiptasia sp.
EGCase
-
 Entacmaea sp.
EGCase
-
 Cellana sp.
EGCase
-
 Meretrix sp.
EGCase
-
 Ruditapes sp.
EGCase
-
 Marphysa sp.
endoglycoceramidase
-
 Bacillus sp. (in: Bacteria)
endoglycoceramidase
-
 Cyanea nozakii
endoglycoceramidase
-
 Hydra vulgaris
endoglycoceramidase
-
 Aiptasia sp.
endoglycoceramidase
-
 Entacmaea sp.
endoglycoceramidase
-
 Cellana sp.
endoglycoceramidase
-
 Meretrix sp.
endoglycoceramidase
-
 Ruditapes sp.
endoglycoceramidase
-
 Marphysa sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4
-
 Bacillus sp. (in: Bacteria)
3 4
-
 Cyanea nozakii
3 4
-
 Hydra vulgaris
3 4
-
 Aiptasia sp.
3 4
-
 Entacmaea sp.
3 4
-
 Cellana sp.
3 4
-
 Meretrix sp.
3 4
-
 Ruditapes sp.
3 4
-
 Marphysa sp.

pI Value

Organism Comment pI Value Maximum pI Value
Hydra vulgaris calculated from amino acid sequence
-
 5.47