Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lubrol Px | optimal concentration for C9 enzyme: 0.1%, no activation of M-777 enzyme | Rhodococcus sp. | |
Triton X-100 | optimum concentration for C9 and M-777 enzyme: 0.1-0.25% | Rhodococcus sp. |
Cloned (Comment) | Organism |
---|---|
recombinant protein from strain C9 | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 87% inhibition at 5 mM | Rhodococcus sp. | |
Cu2+ | complete inhibition at 5 mM | Rhodococcus sp. | |
Hg2+ | complete inhibition at 5 mM | Rhodococcus sp. | |
Zn2+ | 22% inhibition at 5 mM | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide | 37°C, pH 5, M-777 enzyme | Rhodococcus sp. | |
0.5 | - |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide | 37°C, pH 5 | Rhodococcus sp. | |
16.5 | - |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide | 37°C, pH 5, C9 enzyme | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Rhodococcus sp. | - |
M-777 | - |
Rhodococcus sp. C9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM1 | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM1 | Rhodococcus sp. C9 | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GD1b | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | good substrate for strain M-777, weak substrate for strain C9 | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GD1b | Rhodococcus sp. C9 | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | good substrate for strain M-777, weak substrate for strain C9 | Rhodococcus sp. C9 | Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. asialo-GM1 | Rhodococcus sp. | Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. asialo-GM1 | Rhodococcus sp. C9 | Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide | - |
? | |
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. lactosylceramide | Rhodococcus sp. | Galbeta(1-4)Glc + ceramide | - |
? | |
GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | - |
Rhodococcus sp. | GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
additional information | enzyme from strain C9 hydrolyzes b-series gangliotetraosylceramides more slowly than M-777 enzyme, whereas both enzymes hydrolyse a-series gangliosides and neutral glycosphingolipids to the same extent | Rhodococcus sp. | ? | - |
? | |
additional information | enzyme from strain C9 hydrolyzes b-series gangliotetraosylceramides more slowly than M-777 enzyme, whereas both enzymes hydrolyse a-series gangliosides and neutral glycosphingolipids to the same extent | Rhodococcus sp. C9 | ? | - |
? | |
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GD1a | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | good substrate for strain M-777, weak substrate for strain C9 | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GT1b | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | i.e. GM3 | Rhodococcus sp. | NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | good substrate for strain M-777, weak substrate for strain C9 | Rhodococcus sp. | NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GQ1b | Rhodococcus sp. | NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide | - |
? | |
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O | GD3 | Rhodococcus sp. | NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Rhodococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Rhodococcus sp. |