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Literature summary for 3.2.1.123 extracted from

  • Sakaguchi, K.; Okino, N.; Sueyoshi, N.; Izu, H.; Ito, M.
    Cloning and expression of gene encoding a novel endoglycoceramidase of Rhodococcus sp. strain C9 (2000), J. Biochem., 128, 145-152.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Lubrol Px optimal concentration for C9 enzyme: 0.1%, no activation of M-777 enzyme Rhodococcus sp.
Triton X-100 optimum concentration for C9 and M-777 enzyme: 0.1-0.25% Rhodococcus sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein from strain C9 Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 87% inhibition at 5 mM Rhodococcus sp.
Cu2+ complete inhibition at 5 mM Rhodococcus sp.
Hg2+ complete inhibition at 5 mM Rhodococcus sp.
Zn2+ 22% inhibition at 5 mM Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide 37°C, pH 5, M-777 enzyme Rhodococcus sp.
0.5
-
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide 37°C, pH 5 Rhodococcus sp.
16.5
-
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide 37°C, pH 5, C9 enzyme Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, SDS-PAGE Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp.
-
M-777
-
Rhodococcus sp. C9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM1 Rhodococcus sp. C9 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD1b Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O good substrate for strain M-777, weak substrate for strain C9 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD1b Rhodococcus sp. C9 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O good substrate for strain M-777, weak substrate for strain C9 Rhodococcus sp. C9 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. asialo-GM1 Rhodococcus sp. C9 Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
-
?
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. lactosylceramide Rhodococcus sp. Galbeta(1-4)Glc + ceramide
-
?
GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
-
Rhodococcus sp. GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
additional information enzyme from strain C9 hydrolyzes b-series gangliotetraosylceramides more slowly than M-777 enzyme, whereas both enzymes hydrolyse a-series gangliosides and neutral glycosphingolipids to the same extent Rhodococcus sp. ?
-
?
additional information enzyme from strain C9 hydrolyzes b-series gangliotetraosylceramides more slowly than M-777 enzyme, whereas both enzymes hydrolyse a-series gangliosides and neutral glycosphingolipids to the same extent Rhodococcus sp. C9 ?
-
?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD1a Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O good substrate for strain M-777, weak substrate for strain C9 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GT1b Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O i.e. GM3 Rhodococcus sp. NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O good substrate for strain M-777, weak substrate for strain C9 Rhodococcus sp. NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GQ1b Rhodococcus sp. NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
-
?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O GD3 Rhodococcus sp. NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
-
?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Rhodococcus sp.