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Literature summary for 3.2.1.122 extracted from

  • Yip, V.L.; Thompson, J.; Withers, S.G.
    Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4 (2007), Biochemistry, 46, 9840-9852.
    View publication on PubMed
Search for more literature for 3.2.1.122

Inhibitors

Inhibitors Comment Organism Structure
cellobiose 6'-phosphate competitive inhibitor Bacillus subtilis
methyl 6-phospho-alpha-D-glucoside competitive inhibitor Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.012
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
0.014
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.038
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.052
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
0.069
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
0.36
-
 maltose 6'-phosphate at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.4
-
 maltose 6'-phosphate at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
0.5
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.61
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis 3,4-dinitrophenol + alpha-D-glucose 6-phosphate
-
 ?
4-nitrophenyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis 4-nitrophenol + alpha-D-glucose
-
 ?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Bacillus subtilis 4-nitrophenol + beta-D-glucose 6-phosphate
-
 ?
maltose 6'-phosphate + H2O
-
 Bacillus subtilis D-glucose 6-phosphate + D-glucose
-
 ?
methyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis methanol + alpha-D-glucose 6-phosphate
-
 ?
phenyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis phenol + alpha-D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
6-phospho-alpha-glucosidase
-
 Bacillus subtilis
GlvA
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.42
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
0.7
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
0.72
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
0.81
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.85
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
0.9
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
1.1
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
1.3
-
 maltose 6'-phosphate at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
1.3
-
 maltose 6'-phosphate at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
additional information not activated by NADH Bacillus subtilis
NAD+
-
 Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
 cellobiose 6'-phosphate
-
 Bacillus subtilis
0.075
-
 methyl 6-phospho-alpha-D-glucoside
-
 Bacillus subtilis