Cloned (Comment) | Organism |
---|---|
glvA gene, formerly glv1, is encoded within the maltose PEP-PTS operon, it is cloned and overexpressed in Escherichia coli, glvA encodes a 449 amino acid protein | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
recombinant GlvA expressed in Escherichia coli, vapour-phase diffusion using the hanging-drop method, NAD/Mn2+-dependent crystal form | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | requirement, permits formation of the active tetramer of GlvA from its inactive dimeric state | Bacillus subtilis | |
additional information | requires a divalent metal ion, e.g. Mn2+ | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50513 | - |
4 * 50513, native GlvA is active as tetramer | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose 6'-phosphate + H2O | Bacillus subtilis | enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system | D-glucose 6-phosphate + D-glucose | - |
? | |
maltose 6'-phosphate + H2O | Bacillus subtilis GlvA | enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system | D-glucose 6-phosphate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
GlvA | - |
Bacillus subtilis GlvA | - |
GlvA | - |
Purification (Comment) | Organism |
---|---|
recombinant GlvA expressed in Escherichia coli | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose 6'-phosphate + H2O | - |
Bacillus subtilis | D-glucose 6-phosphate + D-glucose | - |
? | |
maltose 6'-phosphate + H2O | enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system | Bacillus subtilis | D-glucose 6-phosphate + D-glucose | - |
? | |
maltose 6'-phosphate + H2O | - |
Bacillus subtilis GlvA | D-glucose 6-phosphate + D-glucose | - |
? | |
maltose 6'-phosphate + H2O | enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system | Bacillus subtilis GlvA | D-glucose 6-phosphate + D-glucose | - |
? | |
trehalose 6-phosphate + H2O | - |
Bacillus subtilis | ? | - |
? | |
trehalose 6-phosphate + H2O | - |
Bacillus subtilis GlvA | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | inactive state | Bacillus subtilis |
tetramer | 4 * 50513, native GlvA is active as tetramer | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
GlvA | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | requires a divalent metal ion, e.g. Mn2+ | Bacillus subtilis | |
NADH | requirement | Bacillus subtilis |