Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Penicillium aurantiogriseum | - |
- |
extracellular | - |
Mucor circinelloides | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58800 | - |
PG I, SDS-PAGE | Penicillium aurantiogriseum |
66400 | - |
PG II, SDS-PAGE | Penicillium aurantiogriseum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-prunasin + H2O | Penicillium aurantiogriseum | - |
mandelonitrile + D-glucose | - |
? | |
(R)-prunasin + H2O | Mucor circinelloides | - |
mandelonitrile + D-glucose | - |
? | |
(R)-prunasin + H2O | Prunus serotina | involved in cyanogenesis from (R)-amygdalin | mandelonitrile + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucor circinelloides | - |
- |
- |
Penicillium aurantiogriseum | - |
- |
- |
Prunus serotina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Penicillium aurantiogriseum |
- |
Mucor circinelloides |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-prunasin + H2O = D-glucose + mandelonitrile | extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity, prunasin hydrolysis is much slower than amygdalin hydrolysis | Penicillium aurantiogriseum | |
(R)-prunasin + H2O = D-glucose + mandelonitrile | extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity, prunasin hydrolysis is much slower than amygdalin hydrolysis | Mucor circinelloides | |
(R)-prunasin + H2O = D-glucose + mandelonitrile | two isoforms found, PG I and PG II | Mucor circinelloides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.26 | - |
purified PG I, pH 6, 25°C | Penicillium aurantiogriseum |
1.11 | - |
purified PG II, pH 6, 25°C | Penicillium aurantiogriseum |
12.26 | - |
purified enzyme, pH 6, 25°C | Mucor circinelloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-prunasin + H2O | - |
Prunus serotina | D-glucose + mandelonitrile | - |
? | |
(R)-prunasin + H2O | slow reaction | Penicillium aurantiogriseum | D-glucose + mandelonitrile | - |
? | |
(R)-prunasin + H2O | slow reaction | Mucor circinelloides | D-glucose + mandelonitrile | - |
? | |
(R)-prunasin + H2O | - |
Penicillium aurantiogriseum | mandelonitrile + D-glucose | - |
? | |
(R)-prunasin + H2O | - |
Mucor circinelloides | mandelonitrile + D-glucose | - |
? | |
(R)-prunasin + H2O | involved in cyanogenesis from (R)-amygdalin | Prunus serotina | mandelonitrile + D-glucose | - |
? |