Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Microbulbifer thermotolerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 53% residual activity at 10 mM | Microbulbifer thermotolerans | |
Cu2+ | 19% residual activity at 10 mM | Microbulbifer thermotolerans | |
EDTA | 23% residual activity at 10 mM | Microbulbifer thermotolerans | |
Fe3+ | 19% residual activity at 10 mM | Microbulbifer thermotolerans | |
Hg2+ | 17% residual activity at 10 mM | Microbulbifer thermotolerans | |
Ni2+ | 77% residual activity at 10 mM | Microbulbifer thermotolerans | |
Zn2+ | 18% residual activity at 10 mM | Microbulbifer thermotolerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Mg2+, Ca2+, Cs2+, Li+, and Mn2+ | Microbulbifer thermotolerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Microbulbifer thermotolerans |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 88% activity at 20% (v/v) | Microbulbifer thermotolerans |
benzene | 93% activity at 20% (v/v) | Microbulbifer thermotolerans |
chloroform | 29% activity at 20% (v/v) | Microbulbifer thermotolerans |
Ethanol | 61% activity at 20% (v/v) | Microbulbifer thermotolerans |
hexane | the enzyme exhibits extreme stability toward 20% (v/v) hexane (99% activity) | Microbulbifer thermotolerans |
Methanol | 56% activity at 20% (v/v) | Microbulbifer thermotolerans |
n-Butanol | 51% activity at 20% (v/v) | Microbulbifer thermotolerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbulbifer thermotolerans | A0A0A0Q4S7 | - |
- |
Microbulbifer thermotolerans DAU221 | A0A0A0Q4S7 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tag affinity column chromatography | Microbulbifer thermotolerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | the Km and Vmax values for the hydrolysis of soluble starch are 1.08 mg/ml and 1.736 mM maltotriose/mg protein/min, respectively | Microbulbifer thermotolerans | maltotriose + ? | - |
? | |
soluble starch + H2O | the Km and Vmax values for the hydrolysis of soluble starch are 1.08 mg/ml and 1.736 mM maltotriose/mg protein/min, respectively | Microbulbifer thermotolerans DAU221 | maltotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Microbulbifer thermotolerans |
Synonyms | Comment | Organism |
---|---|---|
AmyA | - |
Microbulbifer thermotolerans |
maltotriose-producing alpha-amylase | - |
Microbulbifer thermotolerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in sodium phosphate buffer (pH 6.0) | Microbulbifer thermotolerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | at 30°C, the enzyme maintains approximately above 90% activity until 6 h and 80% activity until 12 h. At 40°C, the enzyme maintains approximately above 90% activity until 2 h, 80% activity until 4 h, 70% activity until 8 h, and 60% activity until 12 h. At 50°C, the enzyme maintains approximately 50% activity until 1 h, 30% activity until 6 h, and 20% activity until 12 h | Microbulbifer thermotolerans |