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Literature summary for 3.2.1.114 extracted from
- Cloning, expression, and characterization of Capra hircus Golgi alpha-mannosidase II (2015), Appl. Biochem. Biotechnol., 177, 1241-1251 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed it in Pichia pastoris expression system | Capra hircus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 2 mM, strong inhibition | Capra hircus |  |
| Cu2+ | 2 mM, strong inhibition | Capra hircus | |
| EDTA | 2 mM, strong inhibition | Capra hircus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Capra hircus | 5794 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, about 1.2fold activation | Capra hircus | |
| Fe2+ | 2 mM, about 1.7fold activation | Capra hircus | |
| Mn2+ | 2 mM, about 1.2fold activation | Capra hircus | |
| Zn2+ | 2 mM, about 1.2fold activation | Capra hircus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130500 | - |
calculated from sequence | Capra hircus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capra hircus | V9XY47 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Capra hircus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | low expression | Capra hircus | - |
| liver | - |
Capra hircus | - |
| lung | - |
Capra hircus | - |
| ovary | - |
Capra hircus | - |
| spleen | GMII is the highest expressed gene in the spleen | Capra hircus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-mannopyranoside + H2O | - |
Capra hircus | 4-nitrophenol + D-mannopyranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GmII | - |
Capra hircus |
| Golgi alpha-mannosidase II | - |
Capra hircus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Capra hircus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | 30°C: about 40% of maximal activity, 55°C: about 40% of maximal activity | Capra hircus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Capra hircus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 6.6 | pH 4.2: about 20% of maximal activity, pH 6.6: about 85% of maximal activity | Capra hircus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Capra hircus | calculated from sequence | - |
8.04 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | key glycosyl hydrolase in the N-linked glycosylation pathway | Capra hircus |
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