Application | Comment | Organism |
---|---|---|
drug development | the enzyme Golgi alpha-mannosidase II is a promising target for intervention in the glycosylation process | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
enzyme with bound unhibitors, X-ray diffraction structure determination and analysis | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1S,2R,5R,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol | - |
Drosophila melanogaster | |
(1S,2R,5S,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol | - |
Drosophila melanogaster | |
1-(4-methylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | - |
Drosophila melanogaster | |
1-(4-tert-butylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | - |
Drosophila melanogaster | |
1-(4-tert-butylphenyl)-2-[(1S,2R,5S,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | - |
Drosophila melanogaster | |
1-phenyl-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | - |
Drosophila melanogaster | |
additional information | inhibitor synthesis, overview | Drosophila melanogaster | |
swainsonine | - |
Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Drosophila melanogaster | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | GMII is involved in the creation of glycoproteins that contain complex carbohydrates. It is responsible for the formation of the core trimannose structure to which all complex carbohydrates are appended. It catalyses the hydrolysis of an alpha(1,6)- and an alpha(1,3)-linked mannose from GlcNAc-Man5-GlcNAc2 to form GlcNAc-Man3-GlcNAc2-Asn-X | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q24451 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | GMII is involved in the creation of glycoproteins that contain complex carbohydrates. It is responsible for the formation of the core trimannose structure to which all complex carbohydrates are appended. It catalyses the hydrolysis of an alpha(1,6)- and an alpha(1,3)-linked mannose from GlcNAc-Man5-GlcNAc2 to form GlcNAc-Man3-GlcNAc2-Asn-X | Drosophila melanogaster | ? | - |
? | |
additional information | the cleavage mechanism involves formation of a covalent glycosyl-enzyme intermediate and results in net retention of configuration | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GmII | - |
Drosophila melanogaster |
Golgi alpha-mannosidase II | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.75 | - |
assay at | Drosophila melanogaster |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
1-(4-methylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | pH 5.75, 25°C | Drosophila melanogaster | |
0.0027 | - |
1-(4-tert-butylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | pH 5.75, 25°C | Drosophila melanogaster | |
0.0028 | - |
1-phenyl-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | pH 5.75, 25°C | Drosophila melanogaster | |
0.003 | - |
swainsonine | pH 5.75, 25°C | Drosophila melanogaster |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000029 | - |
pH 5.75, 25°C | Drosophila melanogaster | 1-(4-methylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | |
0.000029 | - |
pH 5.75, 25°C | Drosophila melanogaster | 1-(4-tert-butylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | |
0.00003 | - |
pH 5.75, 25°C | Drosophila melanogaster | 1-phenyl-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | |
0.000037 | - |
pH 5.75, 25°C | Drosophila melanogaster | swainsonine | |
0.000044 | - |
pH 5.75, 25°C | Drosophila melanogaster | (1S,2R,5S,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol | |
0.00025 | - |
pH 5.75, 25°C | Drosophila melanogaster | 1-(4-tert-butylphenyl)-2-[(1S,2R,5S,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone | |
0.00025 | - |
pH 5.75, 25°C | Drosophila melanogaster | (1S,2R,5R,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol |