Literature summary for 3.2.1.114 extracted from

Kuntz, D.A.; Zhong, W.; Guo, J.; Rose, D.R.; Boons, G.J.
The molecular basis of inhibition of Golgi alpha-mannosidase II by mannostatin A (2009), ChemBioChem, 10, 268-277.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Drosophila melanogaster
in complex with inhibitor mannostatin A and its derivatives. The interaction with the backbone carbonyl of residue R876 is crucial to the high potency of the inhibitor and enhanced by the hydrophobic interaction between the thiomethyl group and an aromatic pocket vivinal to the cleavage site	Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism	Structure
(1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol	-	Drosophila melanogaster
(1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetrol	-	Drosophila melanogaster
(1R,2S,3R,4R,5R)-5-aminocyclopentane-1,2,3,4-tetraol	-	Drosophila melanogaster
1-deoxyaminocyclopentitetrol	poor inhibitor	Drosophila melanogaster
Mannostatin A	strong inhibitor, reversible, competitive	Drosophila melanogaster
mannostatin B	strong inhibitor, reversible, competitive	Drosophila melanogaster

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Drosophila melanogaster	5794	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-methylumbelliferyl alpha-D-mannopyranoside + H2O	Drosophila melanogaster	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-
Drosophila melanogaster	Q24451	isoform GMII	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-mannopyranoside + H2O	-	Drosophila melanogaster	?	-	?

Synonyms

Synonyms	Comment	Organism
GmII	-	Drosophila melanogaster
Golgi alpha-mannosidase II	-	Drosophila melanogaster
mannose-trimming enzyme	-	Drosophila melanogaster
mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase II	-	Drosophila melanogaster

pH Range

pH Minimum	pH Maximum	Comment	Organism
3.8	9	30 min	Drosophila melanogaster

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000036	-	Mannostatin A	-	Drosophila melanogaster
0.000076	-	(1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol	-	Drosophila melanogaster
0.00015	-	mannostatin B	-	Drosophila melanogaster
0.0003	-	(1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetrol	-	Drosophila melanogaster
0.0003	-	(1R,2S,3R,4R,5R)-5-aminocyclopentane-1,2,3,4-tetraol	-	Drosophila melanogaster
0.265	-	1-deoxyaminocyclopentitetrol	-	Drosophila melanogaster

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Release 2023.1 (January 2023)