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Literature summary for 3.2.1.114 extracted from

  • Woo, K.K.; Kimura, Y.
    Regulation of substrate specificity of plant alpha-mannosidase by cobalt ion: in vitro hydrolysis of high-mannose type N-glycans by Co2+-activated Ginkgo alpha-mannosidase (2005), Biosci. Biotechnol. Biochem., 69, 1111-1119.
    View publication on PubMed
Search for more literature for 3.2.1.114

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, responsible for regulation of substrate specificity Ginkgo biloba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 x * 120000, SDS-PAGE Ginkgo biloba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ginkgo biloba Co2+ regulates the substrate specificity, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ginkgo biloba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from mature seeds to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration Ginkgo biloba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Ginkgo biloba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-mannoside + H2O
-
 Ginkgo biloba 4-nitrophenol + alpha-D-mannose
-
 ?
Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
additional information Co2+ regulates the substrate specificity, overview Ginkgo biloba ?
-
 ?
additional information in vitro hydrolysis of high-mannose type N-glycans, removal of terminal alpha-1,2-mannose residues, substrate specificity and isomeric substrate/product structures, overview Ginkgo biloba ?
-
 ?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Ginkgo biloba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ginkgo biloba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Ginkgo biloba