Any feedback?
Literature summary for 3.2.1.114 extracted from
- Insect cells encode a class II alpha-mannosidase with unique properties (2001), J. Biol. Chem., 276, 16335-16340.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| alpha-mannosidase III cDNA is cloned | Spodoptera frugiperda |
General Stability
| General Stability | Organism |
|---|---|
| freezing inactivates column-bound glutathione S-transferase-tagged secreted form of SfManIII | Spodoptera frugiperda |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | alpha-mannosidase III, 1 mM, 90% inhibition of 4-nitrophenyl alpha-D-mannoside hydrolysis, 5 mM, complete inhibition of (Man)5(GlcNAc)2-PA hydrolysis | Spodoptera frugiperda |  |
| swainsonine | alpha-mannosidase III, IC50: 10 nM | Spodoptera frugiperda | |
| Zn2+ | alpha-mannosidase III | Spodoptera frugiperda | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | alpha-mannosidase III, integral membrane glycoprotein with type II topology | Spodoptera frugiperda | 139 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation | Spodoptera frugiperda | |
| Co2+ | alpha-mannosidase III, cobalt-dependent | Spodoptera frugiperda | |
| Mn2+ | alpha-mannosidase III, activates to a lesser extend than Co2+ | Spodoptera frugiperda | |
| Ni2+ | alpha-mannosidase III, little activation | Spodoptera frugiperda | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
x * 150000, intact glutathione S-transferase-tagged secreted form of SfManIII, SDS-PAGE | Spodoptera frugiperda |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Spodoptera frugiperda | alpha-mannosidase III probably provides an alternate N-glycan processing pathway in Sf-9 cells | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spodoptera frugiperda | - |
Sf-9, alpha-mannosidase III | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | integral membrane glycoprotein | Spodoptera frugiperda |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione S-transferase-tagged secreted form of SfManIII | Spodoptera frugiperda |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, column-bound glutathione S-transferase-tagged secreted form of SfManIII, at least 1 week, stable | Spodoptera frugiperda |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-mannoside + H2O | alpha-mannosidase III | Spodoptera frugiperda | 4-nitrophenol + alpha-D-mannose | - |
? | |
| additional information | alpha-mannosidase III hydrolyzes (Man)5-9(GlcNAc)2, not: GlcNAc(Man)5(GlcNAc)2 | Spodoptera frugiperda | ? | - |
? | |
| additional information | alpha-mannosidase III probably provides an alternate N-glycan processing pathway in Sf-9 cells | Spodoptera frugiperda | ? | - |
? | |
| pyridylaminated (Man)5(GlcNAc)2 + H2O | via a (Man)4(GlcNAc)2 intermediate | Spodoptera frugiperda | pyridylaminated (Man)3(GlcNAc)2 + alpha-D-mannose | - |
? | |
| pyridylaminated (Man)5(GlcNAc)2 + H2O | alpha-mannosidase III | Spodoptera frugiperda | pyridylaminated (Man)3(GlcNAc)2 + alpha-D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 150000, intact glutathione S-transferase-tagged secreted form of SfManIII, SDS-PAGE | Spodoptera frugiperda |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Spodoptera frugiperda |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 6.7 | - |
Spodoptera frugiperda |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.7 | alpha-mannosidase III activity is negligible at pH 5.5, increases at pH 5.7-5.9, decreases at pH 6.1 and rises to a plateau at pH 6.5-6.7 | Spodoptera frugiperda |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00001 | - |
alpha-mannosidase III, IC50: 10 nM | Spodoptera frugiperda | swainsonine | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
