Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Vigna radiata | |
Triton X-100 | omission results in 40% decrease of activity | Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 20 mM, 90% inhibition | Bombyx mori | |
Cu2+ | - |
Spodoptera frugiperda | |
Cu2+ | - |
Vigna radiata | |
EDTA | 20 mM, 20% inhibition | Bombyx mori | |
EDTA | - |
Spodoptera frugiperda | |
additional information | not inhibited by Ca2+; not inhibited by Mn2+, Mg2+ | Bombyx mori | |
additional information | - |
Spodoptera frugiperda | |
additional information | not inhibited by Mn2+, Mg2+ | Vigna radiata | |
swainsonine | 30 nM, 85% inhibition | Bombyx mori | |
swainsonine | - |
Spodoptera frugiperda | |
swainsonine | - |
Vigna radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound, predominantly in the microsomal pellet | Bombyx mori | 16020 | - |
microsome | - |
Vigna radiata | - |
- |
microsome | predominantly, membrane-bound | Bombyx mori | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Bombyx mori | |
additional information | no metal ion requirement | Vigna radiata | |
additional information | no metal ion requirement | Spodoptera frugiperda | |
additional information | not activated by Ca2+ | Bombyx mori | |
additional information | not activated by Ca2+ | Vigna radiata | |
additional information | not activated by Ca2+ | Spodoptera frugiperda | |
additional information | not activated by Mg2+, Mn2+ | Bombyx mori | |
additional information | not activated by Mg2+, Mn2+ | Vigna radiata | |
additional information | not activated by Mg2+, Mn2+ | Spodoptera frugiperda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Bombyx mori Bm-N | - |
- |
- |
Mamestra brassicae | - |
- |
- |
Mamestra brassicae Mb-0503 | - |
- |
- |
Spodoptera frugiperda | - |
Sf-21 | - |
Spodoptera frugiperda Sf-21 | - |
Sf-21 | - |
Vigna radiata | - |
mung bean | - |
Xenopus laevis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | frog | Xenopus laevis | - |
seedling | - |
Vigna radiata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-mannoside + H2O | - |
Bombyx mori | 4-nitrophenol + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | - |
Vigna radiata | 4-nitrophenol + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | - |
Spodoptera frugiperda Sf-21 | 4-nitrophenol + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | - |
Bombyx mori Bm-N | 4-nitrophenol + alpha-D-mannose | - |
? | |
additional information | - |
Vigna radiata | ? | - |
? | |
additional information | not: (Man)5(GlcNAc)2 | Bombyx mori | ? | - |
? | |
additional information | not: (Man)5(GlcNAc)2 | Spodoptera frugiperda | ? | - |
? | |
additional information | not: (Man)5(GlcNAc)2 | Spodoptera frugiperda Sf-21 | ? | - |
? | |
additional information | not: (Man)5(GlcNAc)2 | Bombyx mori Bm-N | ? | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Spodoptera frugiperda | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Mamestra brassicae | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Bombyx mori | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Xenopus laevis | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Vigna radiata | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | dependent on the prior action of N-acetylglucosaminyl transferase I on (Man)5(GlcNAc)2 | Bombyx mori | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Spodoptera frugiperda Sf-21 | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Bombyx mori Bm-N | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | dependent on the prior action of N-acetylglucosaminyl transferase I on (Man)5(GlcNAc)2 | Bombyx mori Bm-N | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Mamestra brassicae Mb-0503 | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bombyx mori |
37 | - |
assay at | Vigna radiata |
37 | - |
assay at | Spodoptera frugiperda |
37 | - |
assay at | Mamestra brassicae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Bombyx mori |