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Literature summary for 3.2.1.114 extracted from

  • Forsee, W.T.; Palmer, C.F.; Schutzbach, J.S.
    Purification and characterization of an alpha-1,2-mannosidase involved in processing asparagine-linked oligosaccharides (1989), J. Biol. Chem., 264, 3869-3876.
    View publication on PubMed
Search for more literature for 3.2.1.114

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Oryctolagus cuniculus
BAPTA i.e. 1,2-bis(2-aminophenoxy) ethane N,N,N',N'-tetraacetic acid Oryctolagus cuniculus
Co2+
-
 Oryctolagus cuniculus
EDTA Ca2+ restores Oryctolagus cuniculus
EGTA ethylene glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid Oryctolagus cuniculus
Mn2+
-
 Oryctolagus cuniculus
additional information no inhibition by Mg2+ Oryctolagus cuniculus
PCMB Ca2+ protects Oryctolagus cuniculus
phosphatidylinositol CaCl2 or NaCl restores Oryctolagus cuniculus
Zn2+
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 glycoprotein IV from ovalbumin pH 6.0 Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Oryctolagus cuniculus 16020
-
microsome
-
 Oryctolagus cuniculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Oryctolagus cuniculus
40000
-
 gel filtration Oryctolagus cuniculus
49200
-
 sedimentation analysis Oryctolagus cuniculus
52000
-
 1 * 52000, SDS-PAGE, under reducing and non-reducing conditions Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, in 10 mM HEPES/NaOH, pH 7.5, 2 mM MgCl2, 0.1% Nonidet-P40, 0.2 M NaCl, less than 10% loss of activity within 3 months Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
high mannose chains of thyroglobulin and phytohemagglutinin-P + H2O thyroglobulin: 70% of alpha1,2-mannose residues accessible Oryctolagus cuniculus ? + D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc Oryctolagus cuniculus (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
 ?
methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O
-
 Oryctolagus cuniculus ? + D-mannose
-
 ?
ovalbumin glycopeptide + H2O glycopeptide IV Oryctolagus cuniculus ? + D-mannose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE, under reducing and non-reducing conditions Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 in the presence of 10 mM CaCl2 Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 above, stable Oryctolagus cuniculus