BRENDA - Enzyme Database
show all sequences of 3.2.1.114

Purification and characterization of a novel alpha-mannosidase from Aspergillus saitoi

Amano, J.; Kobata, A.; J. Biochem. 99, 1645-1654 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
1 mM, 50% inhibition
Aspergillus phoenicis
additional information
not inhibited by EDTA
Aspergillus phoenicis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.25
-
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc
NaBH4 reduced; pH 5, 37°C
Aspergillus phoenicis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
strongly activates, 1 mM, 13fold
Aspergillus phoenicis
additional information
not activated by Ba2+; not activated by Mg2+, Mn2+; not activated by Zn2+
Aspergillus phoenicis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus phoenicis
-
-
-
Purification (Commentary)
Commentary
Organism
1000fold
Aspergillus phoenicis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin
Aspergillus phoenicis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38000
-
-
Aspergillus phoenicis
Storage Stability
Storage Stability
Organism
-20°C or 4°C, 4 months, less than 5% loss of activity
Aspergillus phoenicis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, poor substrate
586942
Aspergillus phoenicis
?
-
-
-
?
D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, good substrate
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too
586942
Aspergillus phoenicis
?
-
-
-
?
GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
?
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced
586942
Aspergillus phoenicis
Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, cleaves the Manalpha(1-6)Man linkage only after its Manalpha(1-3) residue is removed
586942
Aspergillus phoenicis
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced, no product: Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
586942
Aspergillus phoenicis
?
additional information
not: 4-nitrophenyl alpha-mannoside
586942
Aspergillus phoenicis
?
-
-
-
-
additional information
not: D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-4))[GlcNAcbeta(1-4)(GlcNAcbeta(1-2))Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-4)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, all sodium borate reduced
586942
Aspergillus phoenicis
?
-
-
-
-
additional information
substrate specificity, hydrolysis mechanism of branched oligosaccharides
586942
Aspergillus phoenicis
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus phoenicis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Aspergillus phoenicis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.5
6
pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity
Aspergillus phoenicis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
1 mM, 50% inhibition
Aspergillus phoenicis
additional information
not inhibited by EDTA
Aspergillus phoenicis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.25
-
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc
NaBH4 reduced; pH 5, 37°C
Aspergillus phoenicis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
strongly activates, 1 mM, 13fold
Aspergillus phoenicis
additional information
not activated by Ba2+; not activated by Mg2+, Mn2+; not activated by Zn2+
Aspergillus phoenicis
Purification (Commentary) (protein specific)
Commentary
Organism
1000fold
Aspergillus phoenicis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin
Aspergillus phoenicis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
38000
-
-
Aspergillus phoenicis
Storage Stability (protein specific)
Storage Stability
Organism
-20°C or 4°C, 4 months, less than 5% loss of activity
Aspergillus phoenicis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, poor substrate
586942
Aspergillus phoenicis
?
-
-
-
?
D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, good substrate
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too
586942
Aspergillus phoenicis
?
-
-
-
?
GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
?
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced
586942
Aspergillus phoenicis
Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, cleaves the Manalpha(1-6)Man linkage only after its Manalpha(1-3) residue is removed
586942
Aspergillus phoenicis
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced, no product: Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
586942
Aspergillus phoenicis
?
additional information
not: 4-nitrophenyl alpha-mannoside
586942
Aspergillus phoenicis
?
-
-
-
-
additional information
not: D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-4))[GlcNAcbeta(1-4)(GlcNAcbeta(1-2))Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-4)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, all sodium borate reduced
586942
Aspergillus phoenicis
?
-
-
-
-
additional information
substrate specificity, hydrolysis mechanism of branched oligosaccharides
586942
Aspergillus phoenicis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus phoenicis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Aspergillus phoenicis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.5
6
pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity
Aspergillus phoenicis
Other publictions for EC 3.2.1.114
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750270
Suzuki
Intestinal epithelial cell-sp ...
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43
25-39
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751356
Bobovska
Using DFT methodology for mor ...
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J. Mol. Graph. Model.
66
47-57
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749611
Li
Cloning, expression, and char ...
Capra hircus
Appl. Biochem. Biotechnol.
177
1241-1251
2015
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731972
Lu
Effect of swainsonine in Oxytr ...
Rattus norvegicus
J. Agric. Food Chem.
62
7407-7412
2014
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731660
Cheng
-
From natural product-inspired ...
Homo sapiens
Chemistry
8
2600-2604
2013
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731694
Rose
Structure, mechanism and inhib ...
Drosophila melanogaster
Curr. Opin. Struct. Biol.
22
558-562
2012
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714909
Polakova
alpha-D-mannose derivatives as ...
Homo sapiens
Eur. J. Med. Chem.
46
944-952
2011
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6
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707745
Bello
Novel 2-[(benzylamino)methyl]p ...
Homo sapiens
Bioorg. Med. Chem.
18
3320-3334
2010
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8
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708117
Kuntz
Structural investigation of th ...
Drosophila melanogaster
ChemBioChem
11
673-680
2010
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8
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709324
Shashidhara
Conformational and functional ...
Aspergillus fischeri
J. Fluoresc.
20
827-836
2010
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710037
Zhu
Mechanistic insights into a Ca ...
Homo sapiens
Nat. Chem. Biol.
6
125-132
2010
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9
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1
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9
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1
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2
2
710392
Suits
Structure and kinetic investig ...
Streptococcus pyogenes M1 GAS
PLoS ONE
5
e9006
2010
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1
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1
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1
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7
1
1
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1
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1
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1
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7
1
1
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2
1
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1
1
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2
2
715284
Petersen
Molecular mechanism of the gly ...
Drosophila melanogaster
J. Am. Chem. Soc.
132
8291-8300
2010
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697318
Kuntz
The molecular basis of inhibit ...
Drosophila melanogaster
ChemBioChem
10
268-277
2009
-
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9
9
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1
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1
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708612
Li
Class IIC alpha-mannosidase Af ...
Aspergillus fumigatus, Aspergillus fumigatus YJ-407
Glycobiology
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624-632
2009
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709035
Crispin
A human embryonic kidney 293T ...
Homo sapiens
J. Biol. Chem.
284
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2009
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1
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1
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682772
Kumar
Binding of sulfonium-ion analo ...
Drosophila melanogaster
Proteins
71
1484-1496
2008
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690297
Chida
Change in localization of alka ...
Rattus norvegicus
Acta Histochem. Cytochem.
41
1-5
2008
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696214
Kuntz
Structural analysis of Golgi a ...
Drosophila melanogaster
Biochemistry
47
10058-10068
2008
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1
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10
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1
1
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11
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696732
Fiaux
Functionalized pyrrolidine inh ...
Canavalia ensiformis, Drosophila melanogaster, Homo sapiens
Bioorg. Med. Chem.
16
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2008
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698478
Zhong
Probing the substrate specific ...
Drosophila melanogaster
J. Am. Chem. Soc.
130
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2008
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1
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4
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1
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4
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700989
Shah
Golgi alpha-mannosidase II cle ...
Drosophila melanogaster
Proc. Natl. Acad. Sci. USA
105
9570-9575
2008
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1
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679856
Crispin
Disruption of alpha-mannosidas ...
Homo sapiens
FEBS Lett.
581
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2007
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680041
Kuokkanen
Characterization and subcellul ...
Homo sapiens
Glycobiology
17
1084-1093
2007
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2
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1
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1
1
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682766
Englebienne
Evaluation of docking programs ...
Drosophila melanogaster
Proteins
69
160-176
2007
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17
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663538
Nakajima
Crystallization and preliminar ...
Thermotoga maritima
Acta Crystallogr. Sect. F
62
104-105
2006
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665311
Kawatkar
Structural basis of the inhibi ...
Drosophila melanogaster
J. Am. Chem. Soc.
128
8310-8319
2006
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666601
Strasser
Molecular cloning and characte ...
Arabidopsis thaliana
Plant J.
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789-803
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7
1
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1
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672706
Ferrara
Modulation of therapeutic anti ...
Homo sapiens
Biotechnol. Bioeng.
93
851-861
2006
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2
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677900
Kuntz
-
The role of the active site Zn ...
Drosophila melanogaster
Biocatal. Biotransform.
24
55-61
2006
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1
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681767
Akama
N-Glycan structure analysis us ...
Mus musculus
Methods Enzymol.
416
304-314
2006
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682539
Akama
Essential and mutually compens ...
Mus musculus
Proc. Natl. Acad. Sci. USA
103
8983-8988
2006
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664366
Woo
Regulation of substrate specif ...
Ginkgo biloba
Biosci. Biotechnol. Biochem.
69
1111-1119
2005
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1
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664527
Athanasopoulos
The production, purification a ...
Aspergillus phoenicis
Carbohydr. Res.
340
609-617
2005
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5
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1
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664603
Siriwardena
Potent and selective inhibitio ...
Homo sapiens
ChemBioChem
6
845-848
2005
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5
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1
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1
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3
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666958
Kuntz
-
Crystallographic analysis of t ...
Drosophila melanogaster
Tetrahedron Asymmetry
16
25-32
2005
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1
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6
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Woo
Purification and characterizat ...
Ginkgo biloba
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68
2547-2556
2004
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5
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1
1
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1
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6
1
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5
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2
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1
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1
1
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6
1
1
1
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1
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664599
Li
Inhibition of Golgi mannosidas ...
Homo sapiens
Chembiochem
5
1220-1227
2004
-
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1
-
-
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14
-
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1
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1
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14
-
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1
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-
14
14
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-
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1
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-
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1
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-
-
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646602
Shah
Comparison of kifunensine and ...
Drosophila melanogaster
Biochemistry
42
13812-13816
2003
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1
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3
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1
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3
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2
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1
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1
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2
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1
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3
2
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1
1
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1
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2
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1
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646638
Numao
Insights into the mechanism of ...
Drosophila melanogaster
J. Biol. Chem.
278
48074-48083
2003
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1
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3
1
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1
1
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3
2
4
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1
1
1
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5
1
1
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3
1
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646639
Moremen
Golgi alpha-mannosidase II def ...
Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus
Biochim. Biophys. Acta
1573
225-235
2002
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646643
Francis
Biosynthesis and processing of ...
Spodoptera frugiperda
Glycobiology
12
369-377
2002
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1
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1
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3
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1
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1
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2
1
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1
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3
-
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3
-
-
-
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1
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646641
Oh-Eda
Overexpression of the Golgi-lo ...
Homo sapiens
Eur. J. Biochem.
268
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2001
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1
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1
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1
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1
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1
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1
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2
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7
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1
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646644
Kawar
Insect cells encode a class II ...
Spodoptera frugiperda
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276
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2001
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1
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1
1
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-
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1
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-
1
-
-
-
1
1
3
-
-
1
3
1
1
-
-
1
1
-
-
-
1
5
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
646640
Igdoura
alpha-Mannosidases involved in ...
Rattus norvegicus
Eur. J. Cell Biol.
78
441-452
1999
-
-
-
-
-
-
-
-
1
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1
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1
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4
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2
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1
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1
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4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646637
Ren
Purification and properties of ...
Spodoptera frugiperda
Biochem. J.
324
951-956
1997
-
-
-
-
-
-
5
-
1
1
2
1
-
2
-
-
1
-
-
1
1
-
4
1
1
-
-
-
1
-
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-
-
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-
-
-
-
-
-
-
-
-
5
-
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1
1
2
1
-
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1
-
1
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
646633
Foster
Cloning and sequence analysis ...
Drosophila melanogaster, Mus musculus
Gene
154
183-186
1995
-
-
1
-
-
-
-
-
2
-
1
-
-
2
-
-
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1
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2
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1
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2
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1
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1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646636
Altmann
Processing of asparagine-linke ...
Bombyx mori, Bombyx mori Bm-N, Mamestra brassicae, Mamestra brassicae Mb-0503, Spodoptera frugiperda, Spodoptera frugiperda Sf-21, Vigna radiata, Xenopus laevis
Glycoconj. J.
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1995
2
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11
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3
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12
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-
2
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-
20
-
4
-
-
-
1
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-
2
-
-
-
-
-
-
-
11
-
-
3
3
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-
-
-
-
2
-
-
20
-
4
-
-
-
1
-
-
-
-
-
-
-
-
-
646590
Pan
D-Mannonolactam amidrazone. A ...
Canis lupus familiaris, Vigna radiata
J. Biol. Chem.
267
8313-8318
1992
-
-
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4
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6
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1
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2
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4
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2
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2
4
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2
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1
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2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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135971
Moremen
Novel purification of the cata ...
Rattus norvegicus
J. Biol. Chem.
266
16876-16885
1991
-
-
-
-
-
2
1
1
1
-
4
1
-
1
-
1
1
-
-
1
2
1
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
1
-
4
1
-
-
1
1
-
1
2
1
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646588
Elbein
Glycosidase inhibitors: inhibi ...
plant, Rattus norvegicus
FASEB J.
5
3055-3063
1991
-
-
-
-
-
-
4
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2
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-
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1
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1
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-
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4
-
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-
-
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646631
Moremen
Isolation, characterization, a ...
Homo sapiens, Mus musculus, Rattus norvegicus
J. Cell Biol.
115
1521-1534
1991
-
1
2
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3
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3
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1
3
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3
-
3
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-
-
5
2
-
8
3
-
-
-
-
3
-
-
-
-
-
-
-
1
2
-
-
-
-
-
3
-
-
3
-
1
3
-
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3
-
-
5
2
-
8
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
646626
Kaushal
Purification to homogeneity an ...
Vigna radiata
Biochemistry
29
2168-2176
1990
1
-
-
-
-
1
5
-
1
1
1
-
-
2
-
1
1
-
-
2
1
2
8
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
5
-
-
1
1
1
-
-
-
1
1
-
2
1
2
8
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
646629
Tropea
Mannostatin A, a new glycoprot ...
Canis lupus familiaris, Vigna radiata
Biochemistry
29
10062-10069
1990
-
-
-
-
-
-
5
-
-
-
-
1
-
7
-
-
-
-
-
2
-
-
5
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
5
-
-
-
-
-
1
-
-
-
-
-
2
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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646630
Baron
Mannosidase II and the 135-kDa ...
Rattus norvegicus
J. Biol. Chem.
265
19928-19931
1990
-
-
-
-
-
-
-
-
2
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1
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-
1
-
-
-
-
-
2
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
2
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
646582
Kaushal
Glycoprotein processing enzyme ...
Vigna radiata
Methods Enzymol.
179
452-475
1989
1
-
-
-
-
1
4
-
1
-
1
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1
-
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1
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-
1
1
1
5
1
1
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
-
4
-
-
1
-
1
-
-
-
-
1
-
1
1
1
5
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
646581
Kobata
alpha-Mannosidases I and II fr ...
Aspergillus phoenicis
Methods Enzymol.
138
779-785
1987
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
1
1
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
1
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
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586942
Amano
Purification and characterizat ...
Aspergillus phoenicis
J. Biochem.
99
1645-1654
1986
-
-
-
-
-
-
2
1
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2
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1
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1
-
-
1
1
1
10
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
2
-
-
-
-
-
1
-
1
1
1
10
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
646623
Moremen
Topology of mannosidase II in ...
Rattus norvegicus
J. Biol. Chem.
261
10945-10951
1986
-
-
-
-
-
1
-
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2
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3
1
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3
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-
1
-
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
3
1
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-
-
1
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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646576
Palamarczyk
1,4-Dideoxy-1,4-imino-D-mannit ...
Canis lupus familiaris
Arch. Biochem. Biophys.
243
35-45
1985
-
-
-
-
-
-
1
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-
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3
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-
-
1
-
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-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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646621
Tulsiani
Marked differences in the swai ...
Rattus norvegicus
Arch. Biochem. Biophys.
236
427-434
1985
-
-
-
-
-
-
1
-
3
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-
-
-
4
-
-
1
-
-
3
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
3
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
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646625
Moremen
Biosynthesis and modification ...
Homo sapiens, Mus musculus, Rattus norvegicus
J. Biol. Chem.
260
6654-6662
1985
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-
-
-
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2
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4
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2
3
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4
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8
1
-
-
5
-
-
6
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
2
3
-
-
8
1
-
5
-
-
6
3
3
-
-
-
-
-
-
-
-
-
-
-
-
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646574
Chandrasekaran
Purification and properties of ...
Homo sapiens
Cancer Res.
44
4059-4068
1984
-
-
-
-
-
-
-
1
1
-
-
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1
-
-
1
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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135998
Tulsiani
alpha-D-Mannosidases of rat li ...
Rattus norvegicus
J. Biol. Chem.
257
3660-3668
1982
-
-
-
-
-
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5
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2
2
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1
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2
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1
-
-
2
1
1
9
-
1
-
2
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
2
2
-
1
-
-
-
1
-
2
1
1
9
-
1
-
2
-
2
-
3
-
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646618
Tulsiani
Swainsonine inhibits the biosy ...
Rattus norvegicus
J. Biol. Chem.
257
7936-7939
1982
-
-
-
-
-
-
1
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1
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1
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1
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1
1
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6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
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1
-
1
1
-
6
-
1
-
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1
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646617
Harpaz
Control of glycoprotein synthe ...
Rattus norvegicus
J. Biol. Chem.
255
4894-4902
1980
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1
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2
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
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-
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1
-
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2
-
1
-
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136013
Tulsiani
Purification and characterizat ...
Rattus norvegicus
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252
3227-3233
1977
7
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1
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1
1
2
2
1
1
-
2
-
1
1
2
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-
1
-
7
-
-
-
-
-
3
-
5
-
1
1
3
2
1
-
-
1
1
-
1
1
2
2
1
1
-
2
-
1
1
2
1
-
-
-
-
-
-