Any feedback?
Literature summary for 3.2.1.114 extracted from
- Purification and characterization of alpha-D-mannosidase from rat liver golgi membranes (1977), J. Biol. Chem., 252, 3227-3233.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activation | Rattus norvegicus |  |
| ATP | 1 mM, 27% activation | Rattus norvegicus | |
| bovine serum albumin | 0.125 mg/l, 35% activation | Rattus norvegicus | |
| CMP | 1 mM, 25% activation | Rattus norvegicus | |
| dithiothreitol | activation | Rattus norvegicus | |
| fucose | 250 mM, 15-20% activation | Rattus norvegicus | |
| additional information | not activated by glucose, galactose, AMP, ADP, GMP, GDP, GTP, CDP, CTP, UMP, UDP, UTP, cAMP, cGMP | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| 4-nitrophenyl alpha-D-mannoside stabilizes | Rattus norvegicus |
| requires presence of detergent, 0.1% Triton X-100, to maintain enzyme in soluble form | Rattus norvegicus |
| stable to repeated freeze-thawing cycles | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong | Rattus norvegicus | |
| Fe2+ | strong | Rattus norvegicus | |
| mannose | 250 mM, 30% inhibition | Rattus norvegicus | |
| additional information | not inhibited by chelating agents, 0.1 mM 1,10-phenanthroline, 1 mM 2,2'-dipyridyl, 250 mM glucose, 250 mM galactose; not inhibited by EDTA; not inhibited by Mn2+, Mg2+ | Rattus norvegicus | |
| p-chloromercuriphenylsulfonate | 1 mM, 80% inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
4-nitrophenyl alpha-D-mannoside | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | - |
Rattus norvegicus | 139 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation | Rattus norvegicus | |
| additional information | not activated by Na+ | Rattus norvegicus | |
| additional information | not activated by Mg2+, Mn2+ | Rattus norvegicus | |
| Zn2+ | activation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
| 295000 | - |
tetrameric form, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | function in Golgi membranes: presumably glycopolymer metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar rats | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | presumably | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 97fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.1 | - |
pH 5.5, 37°C | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C or frozen, 0.1 M potassium phosphate buffer, pH 7.2, Triton X-100, 30 days, 20% loss of activity | Rattus norvegicus |
| frozen, at least 2 months, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Rattus norvegicus | 4-nitrophenol + alpha-D-mannose | - |
? | |
| additional information | function in Golgi membranes: presumably glycopolymer metabolism | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 75000-80000, enzyme exists also in a relatively stable 150 kDa dimeric form, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
stable at pH 7-9, unstable at acidic pH-values, Zn2+ stabilizes | Rattus norvegicus |
| 40 | - |
and below, 15 min stable, rapid inactivation above, Zn2+ or 0.125 mg/l bovine serum albumin stabilizes | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 6 | pH 4.2: about half-maximal activity, pH 6: about 80% of maximal activity | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | unstable at 37°C, no activity below pH 4 | Rattus norvegicus |
| 7 | 9 | stable at 37°C | Rattus norvegicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rattus norvegicus | pH 3 to 10 gradient, isoelectric focusing | - |
5.8 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
