Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.113 extracted from

  • Jin, Z.C.; Kitajima, T.; Dong, W.; Huang, Y.F.; Ren, W.W.; Guan, F.; Chiba, Y.; Gao, X.D.; Fujita, M.
    Genetic disruption of multiple alpha1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans (2018), J. Biol. Chem., 293, 5572-5584 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.113

Application

Application Comment Organism
pharmacology in triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 are the major N-glycan structures. The N-glycan structures on recombinant proteins expressed in triple KO cells are simplified and changed from complex types to high-mannose types at the protein level. The triple KO HEK293 cells are suitable for producing recombinant proteins, including lysosomal enzymes with high-mannose-type N-glycans. This approach should accelerate the production of biopharmaceutical proteins with homogenous glycans Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
Golgi apparatus
-
 Homo sapiens 5794
-

Organism

Organism UniProt Comment Textmining
Homo sapiens O60476
-
-
Homo sapiens P33908
-
-
Homo sapiens Q9UKM7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
endoplasmic reticulum mannosidase I
-
 Homo sapiens
MAN1A1
-
 Homo sapiens
MAN1A2
-
 Homo sapiens
MAN1B1
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction in triple KO (MAN1A1, MAN1A2, and MAN1B1) cells, Man9GlcNAc2 and Man8GlcNAc2 are the major N-glycan structures. Knockout of the MAN1A1 or MAN1A2 gene does not change the glycan profiles. Double KO cells (MAN1A1, MAN1A2) displayed increased high-mannose-type and decreased complex-type glycans. The N-glycan structures on recombinant proteins expressed in triple KO cells are simplified and changed from complex types to high-mannose types at the protein level Homo sapiens