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Literature summary for 3.2.1.113 extracted from
- Cloning and expression of 1,2-alpha-mannosidase gene (fmanIB) from filamentous fungus Aspergillus oryzae: in vivo visualization of the FmanIBp-GFP fusion protein (2006), Biosci. Biotechnol. Biochem., 70, 471-479.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression by an Aspergillus host | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimycin | 0.2 mM, 50% inhibition | Aspergillus oryzae |  |
| EDTA | enzyme loses 96% activity after dialysis in 50 mM PIPES buffer, pH 6.0, containing 2 mM EDTA. Addition of 10 mM Ca2+ recovers activity to 100%, addition of 10 mM Mg2+ recovers activity to 43% | Aspergillus oryzae | |
| kifunensine | 0.0015 mM, 50% inhibition | Aspergillus oryzae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1 | Aspergillus oryzae | - |
- |
| intracellular | with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1 | Aspergillus oryzae | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, completely recovers activity after treatment with EDTA | Aspergillus oryzae | |
| Mg2+ | 10 mM, recovers activity to 43% after treatment with EDTA | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus oryzae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-alpha-D-mannobiose + H2O | - |
Aspergillus oryzae | D-mannose | - |
? | |
| Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | - |
Aspergillus oryzae | (Man)5GlcNAc + 4 D-mannose | - |
? | |
| additional information | no activity with p-nitrophenyl-alpha-mannoside, Manalpha(1-3)Man-OMe or Manalpha(1-6)Man-OMe | Aspergillus oryzae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FmanIBp | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
hydrolysis of 1,2-alpha-mannobiose | Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 60 | 45°C: optimum, 60°C: 60% of maximal activity, 65°C: 14% of maximal activity, hydrolysis of 1,2-alpha-mannobiose | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
hydrolysis of 1,2-alpha-mannobiose | Aspergillus oryzae |
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