Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxymannojirimycin | not significantly | Trypanosoma cruzi | |
D-mannono-delta-lactam | - |
Trypanosoma cruzi | |
D-mannono-gamma-lactone | - |
Trypanosoma cruzi | |
EDTA | strong | Trypanosoma cruzi | |
Fe2+ | strong | Trypanosoma cruzi | |
kifunensine | - |
Trypanosoma cruzi | |
swainsonine | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
p-nitrophenyl-alpha-D-mannoside | 37°C, pH 6.1 | Trypanosoma cruzi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation | Trypanosoma cruzi | |
Ni2+ | activation | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
2 * 76000, SDS-PAGE | Trypanosoma cruzi |
150000 | 160000 | gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
intracellular protozoan, causes Chagas disease in humans, epimastigote stage | - |
Purification (Comment) | Organism |
---|---|
partial | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | - |
Trypanosoma cruzi | (Man)5GlcNAc + 4 D-mannose | product is Man6GlcNAc | ? | |
additional information | no hydrolysis of alpha1,3-linkages | Trypanosoma cruzi | ? | - |
? | |
additional information | no hydrolysis of alpha1,6-linkages | Trypanosoma cruzi | ? | - |
? | |
p-nitrophenyl-alpha-D-mannoside + H2O | - |
Trypanosoma cruzi | 4-nitrophenol + D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 76000, SDS-PAGE | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6.5 | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
swainsonine | pH 6.1, 37°C | Trypanosoma cruzi | |
0.002 | - |
D-mannono-delta-lactam | pH 6.1, 37°C | Trypanosoma cruzi | |
0.006 | - |
kifunensine | pH 6.1, 37°C | Trypanosoma cruzi |