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Literature summary for 3.2.1.113 extracted from

  • Ren, J.; Bretthauer, R.K.; Castellino, F.J.
    Purification and properties of a Golgi-derived (alpha 1,2)-mannosidase-I from baculovirus-infected lepidopteran insect cells (IPLB-SF21AE) with preferential activity toward mannose6-N-acetylglucosamine2 (1995), Biochemistry, 34, 2489-2495.
    View publication on PubMed
Search for more literature for 3.2.1.113

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Spodoptera frugiperda
additional information
-
 Spodoptera frugiperda

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Spodoptera frugiperda 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Spodoptera frugiperda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 x * 63000, SDS-PAGE Spodoptera frugiperda

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda
-
 IPLB-SF-21AE, baculovirus-infected
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O preferred substrate Spodoptera frugiperda (Man)5(GlcNAc)2 + D-mannose
-
 ?
ovalbumin glycopeptide + H2O
-
 Spodoptera frugiperda ? + D-mannose
-
 ?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Spodoptera frugiperda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Spodoptera frugiperda

pH Range

pH Minimum pH Maximum Comment Organism
5.3 6.6 about half-maximal activity at pH 5.3 and 6.6 Spodoptera frugiperda