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Literature summary for 3.2.1.113 extracted from

  • Bause, E.; Breuer, W.; Schweden, J.; Roeser, R.; Geyer, R.
    Effect of substrate structure on the activity of Man9-mannosidase from pig liver involved in N-linked oligosaccharide processing (1992), Eur. J. Biochem., 208, 451-457.
    View publication on PubMed
Search for more literature for 3.2.1.113

General Stability

General Stability Organism
enzyme loses a membrane-spanning domain during purification which does not alter its catalytic properties Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin strong Sus scrofa
additional information
-
 Sus scrofa
N-5-Carboxypentyldeoxymannojirimycin strong Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic constants of various substrate derivatives Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Sus scrofa 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 1 * 49000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Sus scrofa
-
 (Man)5GlcNAc + 4 D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Sus scrofa (Man)5GlcNAc + 4 D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O removes 3 of the 4 alpha1,2-linked mannosyl residues Sus scrofa (Man)6(GlcNAc)2 + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O intact chitobiose core affects Man9-mannosidase specificity: reduction or removal of terminal N-acetylglucosamine residue increases hydrolytic susceptibility of the fourth alpha1,2-mannosyl-linkage Sus scrofa (Man)6(GlcNAc)2 + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-hexapeptide + H2O substrate specificity not influenced by peptide moiety, removes 3 mannose residues Sus scrofa (Man)6(GlcNAc)2-hexapeptide + D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O very poor substrate Sus scrofa (Man)5(GlcNAc)2 + D-mannose
-
 ?
additional information no substrate: 4-methylumbelliferyl-alpha-mannoside Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Sus scrofa