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Literature summary for 3.2.1.113 extracted from

  • Tabas, I.; Kornfeld, S.
    Purification and characterization of a rat liver Golgi alpha-mannosidase capable of processing asparagine-linked oligosaccharides (1979), J. Biol. Chem., 254, 11655-11663.
    View publication on PubMed
Search for more literature for 3.2.1.113

Inhibitors

Inhibitors Comment Organism Structure
Co2+ weak Rattus norvegicus
Cu2+
-
 Rattus norvegicus
EDTA weak Rattus norvegicus
Tris/maleate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Man8GlcNAc 37°C, pH 6.5 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Rattus norvegicus 139
-
membrane
-
 Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Rattus norvegicus
-
 (Man)5GlcNAc + 4 D-mannose and (Man)6GlcNAc ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Rattus norvegicus Sprague-Dawley
-
 (Man)5GlcNAc + 4 D-mannose and (Man)6GlcNAc ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male
-
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4°C, 1 mg protein/ml, in 50 mM phosphate buffer, pH 6.5, 5 mM MgCl2, 0.1% Triton X-100, 30 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man8GlcNAc + H2O
-
 Rattus norvegicus Man7GlcNAc + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Rattus norvegicus (Man)5GlcNAc + 4 D-mannose and (Man)6GlcNAc ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O i.e. (Man)9GlcNAc, specific for alpha-1,2-linked mannose residues Rattus norvegicus (Man)5GlcNAc + 4 D-mannose and (Man)6GlcNAc ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Rattus norvegicus Sprague-Dawley (Man)5GlcNAc + 4 D-mannose and (Man)6GlcNAc ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O i.e. (Man)9GlcNAc, specific for alpha-1,2-linked mannose residues Rattus norvegicus Sprague-Dawley (Man)5GlcNAc + 4 D-mannose and (Man)6GlcNAc ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2))Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-peptide + H2O
-
 Rattus norvegicus (Man)5(GlcNAc)-peptide + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2))Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-peptide + H2O
-
 Rattus norvegicus Sprague-Dawley (Man)5(GlcNAc)-peptide + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
-
 Rattus norvegicus ? + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
-
 Rattus norvegicus Sprague-Dawley ? + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
-
 Rattus norvegicus ? + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
-
 Rattus norvegicus Sprague-Dawley ? + D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
-
 Rattus norvegicus ? + D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O without alpha1,2-linked mannose residues: poor substrate Rattus norvegicus (Man)4GlcNAc + (Man)3GlcNAc + D-mannose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 90 min, about 60-70% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 (Man)8(GlcNAc)2-peptide as substrate Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 about half-maximal activity at pH 5.5 and 7 Rattus norvegicus
5.5 7 (Man)8(GlcNAc)2-peptide as substrate Rattus norvegicus