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Literature summary for 3.2.1.113 extracted from

  • Shigematsu, Y.; Tsukahara, K.; Tanaka, T.; Takeuchi, M.; Ichishima, E.
    Molecular and enzymic properties of 1,2-alpha-D-mannosidase from Aspergillus saitoi (1986), Curr. Microbiol., 13, 43-46.
No PubMed abstract available
Search for more literature for 3.2.1.113

Inhibitors

Inhibitors Comment Organism Structure
1-Cyclohexyl-3-(2-morpholinyl-4-ethyl)carbodiimide/glycine methylester
-
 Aspergillus phoenicis
Ag+ not Aspergillus phoenicis
Co2+
-
 Aspergillus phoenicis
Cu2+
-
 Aspergillus phoenicis
EDTA weak Aspergillus phoenicis
Fe2+
-
 Aspergillus phoenicis
iodoacetic acid monoiodoacetic acid, 1 mM, weak Aspergillus phoenicis
mannosamine not Aspergillus phoenicis
methyl-alpha-mannoside
-
 Aspergillus phoenicis
Mn2+
-
 Aspergillus phoenicis
additional information no inhibition by 4-nitrophenyl-alpha-mannoside, mannitol, L-mannono-1,4-lactone, PMSF Aspergillus phoenicis
PCMB not Aspergillus phoenicis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 mannobiose pH 5.0 Aspergillus phoenicis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Aspergillus phoenicis
64000
-
 x * 64000, SDS-PAGE Aspergillus phoenicis

Organism

Organism UniProt Comment Textmining
Aspergillus phoenicis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 19.4% neutral and 3.9% amino sugars Aspergillus phoenicis

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Aspergillus phoenicis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
baker's yeast mannan + H2O
-
 Aspergillus phoenicis ? + D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O as in Taka-amylase A Aspergillus phoenicis (Man)5(GlcNAc)2 + D-mannose
-
 ?
mannobiose + H2O
-
 Aspergillus phoenicis 2 D-mannose
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Aspergillus phoenicis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aspergillus phoenicis