Literature summary for 3.2.1.111 extracted from

Li, T.; Li, M.; Hou, L.; Guo, Y.; Wang, L.; Sun, G.; Chen, L.
Identification and characterization of a core fucosidase from the bacterium Elizabethkingia meningoseptica (2018), J. Biol. Chem., 293, 1243-1258 .

Search for more literature for 3.2.1.111

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	115.2% activity at 5 mM	Elizabethkingia meningoseptica

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Elizabethkingia meningoseptica

Protein Variants

Protein Variants	Comment	Organism
249DELTA265	the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (14fold) 3-fucosyllactose (5fold) and Lewis X trisaccharide (9fold) compared to the wild type enzyme	Elizabethkingia meningoseptica
C317A	the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (9fold) 3-fucosyllactose (4fold) and Lewis X trisaccharide (6fold) compared to the wild type enzyme	Elizabethkingia meningoseptica
C317S	the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (2fold) 3-fucosyllactose (3fold) and Lewis X trisaccharide (2fold) compared to the wild type enzyme	Elizabethkingia meningoseptica
C467S	the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (0.9fold) 3-fucosyllactose (0.9fold) and Lewis X trisaccharide (9fold) compared to the wild type enzyme	Elizabethkingia meningoseptica
C73S	the mutant shows wild type activity with 4-nitrophenyl alpha-L-fucoside and Lewis X trisaccharide and shows decreased activity with 3-fucosyllactose (2fold) compared to the wild type enzyme	Elizabethkingia meningoseptica
D242A	the mutant is inactive with 3-fucosyllactose and shows decreased activity with 4-nitrophenyl alpha-L-fucoside (10000fold) and Lewis X trisaccharide (1928fold) compared to the wild type enzyme	Elizabethkingia meningoseptica
E302A	the mutant is inactive with 3-fucosyllactose and shows decreased activity with 4-nitrophenyl alpha-L-fucoside (736fold) and Lewis X trisaccharide (900fold) compared to the wild type enzyme	Elizabethkingia meningoseptica
E315A	the mutant is inactive with 4-nitrophenyl alpha-L-fucoside and shows decreased activity with 3-fucosyllactose (473fold) and Lewis X trisaccharide (585fold) compared to the wild type enzyme	Elizabethkingia meningoseptica

Inhibitors

Inhibitors	Comment	Organism
Cu2+	14.6% residual activity at 1 mM	Elizabethkingia meningoseptica
deoxyfuconojirimycin	about 15% residual activity at 0.5 mM	Elizabethkingia meningoseptica
additional information	not inhibited by EDTA, urea or dithiothreitol	Elizabethkingia meningoseptica
p-chloromercuribenzoate	0.5% residual activity at 0.5 mM	Elizabethkingia meningoseptica
SDS	0.1% (w/v) SDS almost inactivates the enzyme	Elizabethkingia meningoseptica
Zn2+	87% residual activity at 1 mM	Elizabethkingia meningoseptica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.5	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.5	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.6	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.6	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.6	-	4-nitrophenyl alpha-L-fucoside	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.2	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme E302A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.3	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.3	-	Lewis X trisaccharide	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.5	-	Lewis X trisaccharide	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
2	-	3-fucosyllactose	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
2.3	-	3-fucosyllactose	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
2.6	-	Lewis X trisaccharide	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
2.8	-	Lewis X trisaccharide	mutant enzyme E315A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
3	-	3-fucosyllactose	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
3.3	-	Lewis X trisaccharide	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
4.1	-	3-fucosyllactose	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
4.1	-	Lewis X trisaccharide	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
5.1	-	3-fucosyllactose	mutant enzyme E315A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
5.3	-	3-fucosyllactose	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
6.9	-	3-fucosyllactose	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
9.7	-	Lewis X trisaccharide	mutant enzyme D242A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
12.8	-	Lewis X trisaccharide	mutant enzyme E302A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
13.2	-	Lewis X trisaccharide	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
17.4	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme D242A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	Mg2+, Mn2+, Ca2+, Ni2+, Fe2+, and Cd2+ have no significant effect on the enzymatic activity	Elizabethkingia meningoseptica

Organism

Organism	UniProt	Comment	Textmining
Elizabethkingia meningoseptica	-	-	-
Elizabethkingia meningoseptica FMS-007	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-Sepharose 6 column chromatography	Elizabethkingia meningoseptica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3-fucosyllactose + H2O	1328.6% activity compared to 4-nitrophenyl alpha-L-fucoside	Elizabethkingia meningoseptica	L-fucose + lactose	-	?
3-fucosyllactose + H2O	1328.6% activity compared to 4-nitrophenyl alpha-L-fucoside	Elizabethkingia meningoseptica FMS-007	L-fucose + lactose	-	?
4-nitrophenyl alpha-L-fucoside + H2O	100% activity	Elizabethkingia meningoseptica	4-nitrophenol + L-fucose	-	?
4-nitrophenyl alpha-L-fucoside + H2O	100% activity	Elizabethkingia meningoseptica FMS-007	4-nitrophenol + L-fucose	-	?
4-nitrophenyl fucoside + H2O	-	Elizabethkingia meningoseptica	4-nitrophenol + L-fucose	-	?
4-nitrophenyl fucoside + H2O	-	Elizabethkingia meningoseptica FMS-007	4-nitrophenol + L-fucose	-	?
horseradish type VI horseradish peroxidase + H2O	-	Elizabethkingia meningoseptica	?	-	?
horseradish type VI horseradish peroxidase + H2O	-	Elizabethkingia meningoseptica FMS-007	?	-	?
Lewis X trisaccharide + H2O	15448.5% activity compared to 4-nitrophenyl alpha-L-fucoside	Elizabethkingia meningoseptica	?	-	?
additional information	no activity with 4-nitrophenyl beta-L-fucoside, 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-lactoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl alpha-D-xyloside, 4-nitrophenyl beta-D-mannoside, 4-nitrophenyl alpha-D-mannoside, 4-nitrophenyl alpha-D-N-acetyl-glucoside, 4-nitrophenyl beta-D-N-acetyl-glucoside, 4-nitrophenyl alpha-D-N-acetyl-galactoside, 4-nitrophenyl beta-D-N-acetyl-galactoside, and 4-nitrophenyl alpha-D-N-acetyl-neuraminide. The enzyme is also inactive against 2'-fucsoyllactose, 6-fucosyl-N-acetylglucosamine, 6-fucosyl-N,N'-diacetylchitobiose, and Lewis A trisaccharide	Elizabethkingia meningoseptica	?	-	?
additional information	no activity with 4-nitrophenyl beta-L-fucoside, 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-lactoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl alpha-D-xyloside, 4-nitrophenyl beta-D-mannoside, 4-nitrophenyl alpha-D-mannoside, 4-nitrophenyl alpha-D-N-acetyl-glucoside, 4-nitrophenyl beta-D-N-acetyl-glucoside, 4-nitrophenyl alpha-D-N-acetyl-galactoside, 4-nitrophenyl beta-D-N-acetyl-galactoside, and 4-nitrophenyl alpha-D-N-acetyl-neuraminide. The enzyme is also inactive against 2'-fucsoyllactose, 6-fucosyl-N-acetylglucosamine, 6-fucosyl-N,N'-diacetylchitobiose, and Lewis A trisaccharide	Elizabethkingia meningoseptica FMS-007	?	-	?
phospholipase A2 + H2O	-	Elizabethkingia meningoseptica	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 52000, SDS-PAGE	Elizabethkingia meningoseptica

Synonyms

Synonyms	Comment	Organism
cFase I	-	Elizabethkingia meningoseptica
core fucosidase I	-	Elizabethkingia meningoseptica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Elizabethkingia meningoseptica

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	60	more than 60% activity between 40 and 60°C	Elizabethkingia meningoseptica

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	the enzyme quickly loses its hydrolytic activity at 55°C	Elizabethkingia meningoseptica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.00037	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme E302A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.0004	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme D242A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.013	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.018	-	3-fucosyllactose	mutant enzyme E315A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.022	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.062	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.078	-	Lewis X trisaccharide	mutant enzyme E315A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.082	-	Lewis X trisaccharide	mutant enzyme D242A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.127	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.14	-	4-nitrophenyl alpha-L-fucoside	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.143	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.23	-	Lewis X trisaccharide	mutant enzyme E302A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.34	-	3-fucosyllactose	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.35	-	3-fucosyllactose	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
3.55	-	3-fucosyllactose	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
3.87	-	3-fucosyllactose	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
3.99	-	3-fucosyllactose	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
4.12	-	3-fucosyllactose	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
9.075	-	Lewis X trisaccharide	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
21.55	-	Lewis X trisaccharide	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
23.83	-	Lewis X trisaccharide	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
25.43	-	Lewis X trisaccharide	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
29.07	-	Lewis X trisaccharide	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
34.47	-	Lewis X trisaccharide	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.6	-	-	Elizabethkingia meningoseptica

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	6	about 85% activity at pH 4.0, about 60% activity at pH 5.0, about 45% activity at pH 6.0	Elizabethkingia meningoseptica

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2.5	3	the enzyme is unstable at pH values less than 3.0	Elizabethkingia meningoseptica

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.000023	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme D242A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.000317	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme E302A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.0033	-	3-fucosyllactose	mutant enzyme E315A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.0083	-	Lewis X trisaccharide	mutant enzyme D242A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.017	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.0183	-	Lewis X trisaccharide	mutant enzyme E302A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.027	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.0283	-	Lewis X trisaccharide	mutant enzyme E315A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.1	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.222	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.232	-	4-nitrophenyl alpha-L-fucoside	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.248	-	4-nitrophenyl alpha-L-fucoside	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.328	-	3-fucosyllactose	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.45	-	3-fucosyllactose	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.512	-	3-fucosyllactose	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
0.723	-	3-fucosyllactose	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.78	-	3-fucosyllactose	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
1.81	-	Lewis X trisaccharide	mutant enzyme 249DELTA265, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
2.035	-	3-fucosyllactose	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
2.77	-	Lewis X trisaccharide	mutant enzyme C317A, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
7.04	-	Lewis X trisaccharide	mutant enzyme C317S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
13.06	-	Lewis X trisaccharide	mutant enzyme C73S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
16.12	-	Lewis X trisaccharide	wild type enzyme, at pH 4.6 and 37°C	Elizabethkingia meningoseptica
17.41	-	Lewis X trisaccharide	mutant enzyme C467S, at pH 4.6 and 37°C	Elizabethkingia meningoseptica