BRENDA - Enzyme Database
show all sequences of 3.2.1.111

Purification of an almond emulsin fucosidase on Cibacron blue-Sepharose and demonstration of its activity toward fucose-containing glycoproteins

Imbert, M.J.; Glasgow, L.R.; Pizzo, S.V.; J. Biol. Chem. 257, 8205-8210 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
decreasing ionic strength inactivates
Prunus dulcis
freeze-thawing results in 30% loss of initial activity
Prunus dulcis
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
strong
Prunus dulcis
L-fucose
-
Prunus dulcis
malonate
above 20 mM
Prunus dulcis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
slight activation
Prunus dulcis
Mg2+
slight activation
Prunus dulcis
additional information
no activation by Mn2+
Prunus dulcis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
gel filtration
Prunus dulcis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Prunus dulcis
-
almond
-
Purification (Commentary)
Commentary
Organism
affinity chromatography on Blue Sepharose; partial
Prunus dulcis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
emulsin
commercially available
Prunus dulcis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0645
-
-
Prunus dulcis
Storage Stability
Storage Stability
Organism
4°C, less than 10% loss of activity within 6 months
Prunus dulcis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
asialoorosomucoid + H2O
specifically hydrolyzes fucose in alpha-1,3-linkage to N-acetylglucosamine of Asn-linked oligosaccharide chain branches
646567
Prunus dulcis
?
-
-
-
?
lactoferrin + H2O
hydrolyzes only alpha-1,3-linkages between fucose and N-acetylglucosamine
646567
Prunus dulcis
?
-
-
-
?
additional information
no substrates are alpha2-macroglobulin (MW 72600), bovine IgG glycopeptides or milk oligosaccharide 2'-fucosyllactose
646567
Prunus dulcis
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Prunus dulcis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
-
-
Prunus dulcis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.3
5.8
about half-maximal activity at pH 4.3 and about 80% of maximal activity at pH 5.8
Prunus dulcis
General Stability (protein specific)
General Stability
Organism
decreasing ionic strength inactivates
Prunus dulcis
freeze-thawing results in 30% loss of initial activity
Prunus dulcis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
strong
Prunus dulcis
L-fucose
-
Prunus dulcis
malonate
above 20 mM
Prunus dulcis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
slight activation
Prunus dulcis
Mg2+
slight activation
Prunus dulcis
additional information
no activation by Mn2+
Prunus dulcis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
gel filtration
Prunus dulcis
Purification (Commentary) (protein specific)
Commentary
Organism
affinity chromatography on Blue Sepharose; partial
Prunus dulcis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
emulsin
commercially available
Prunus dulcis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0645
-
-
Prunus dulcis
Storage Stability (protein specific)
Storage Stability
Organism
4°C, less than 10% loss of activity within 6 months
Prunus dulcis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
asialoorosomucoid + H2O
specifically hydrolyzes fucose in alpha-1,3-linkage to N-acetylglucosamine of Asn-linked oligosaccharide chain branches
646567
Prunus dulcis
?
-
-
-
?
lactoferrin + H2O
hydrolyzes only alpha-1,3-linkages between fucose and N-acetylglucosamine
646567
Prunus dulcis
?
-
-
-
?
additional information
no substrates are alpha2-macroglobulin (MW 72600), bovine IgG glycopeptides or milk oligosaccharide 2'-fucosyllactose
646567
Prunus dulcis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Prunus dulcis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
-
-
Prunus dulcis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.3
5.8
about half-maximal activity at pH 4.3 and about 80% of maximal activity at pH 5.8
Prunus dulcis
Other publictions for EC 3.2.1.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750434
Zeuner
Loop engineering of an alpha- ...
Bifidobacterium bifidum, Clostridium perfringens
Enzyme Microb. Technol.
115
37-44
2018
-
-
2
-
2
-
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751107
Li
Identification and characteri ...
Elizabethkingia meningoseptica, Elizabethkingia meningoseptica FMS-007
J. Biol. Chem.
293
1243-1258
2018
1
-
1
-
8
-
6
21
-
1
-
-
-
2
-
-
1
-
-
-
-
-
12
1
1
1
1
24
1
1
1
-
-
-
-
1
-
1
-
-
8
-
-
6
-
21
-
1
-
-
-
-
-
1
-
-
-
-
12
1
1
1
1
24
1
1
1
-
-
-
-
-
24
24
750709
Saumonneau
Design of an alpha-L-transfuc ...
Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Glycobiology
26
261-269
2016
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708607
Ashida
Two distinct alpha-L-fucosidas ...
Bifidobacterium bifidum
Glycobiology
19
1010-1017
2009
-
-
1
-
1
-
2
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
9
1
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
1
-
-
2
-
-
-
-
2
1
-
-
-
-
-
-
-
-
9
1
1
-
1
-
1
-
1
-
-
1
1
-
1
1
646567
Imbert
Purification of an almond emul ...
Prunus dulcis
J. Biol. Chem.
257
8205-8210
1982
-
-
-
-
-
2
3
-
-
3
1
-
-
1
-
-
1
-
-
1
1
1
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
3
1
-
-
-
-
1
-
1
1
1
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
208728
Yoshima
Purification of almond emulsin ...
Prunus dulcis
Arch. Biochem. Biophys.
194
394-398
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
1
2
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
2
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
208732
Ogata-Arakawa
alpha-L-Fucosidases from almon ...
Prunus dulcis
Arch. Biochem. Biophys.
181
353-358
1977
1
-
-
-
-
1
-
2
-
-
-
1
-
1
-
-
1
-
-
1
1
-
4
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
1
-
1
1
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-