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Literature summary for 3.2.1.111 extracted from
- alpha-L-Fucosidases from almond emulsin: characterization of the two enzymes with different specificities (1977), Arch. Biochem. Biophys., 181, 353-358.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 0.1 M, required for optimal activity | Prunus dulcis |  |
General Stability
| General Stability | Organism |
|---|---|
| major loss of activity by ion-exchange chromatography | Prunus dulcis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.095 | - |
lacto-N-fucopentaitol III | - |
Prunus dulcis | |
| 0.101 | - |
lacto-N-fucopentaitol II | pH 5.0, 37°C | Prunus dulcis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Prunus dulcis | the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prunus dulcis | - |
almond | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Prunus dulcis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| emulsin | - |
Prunus dulcis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00146 | - |
- |
Prunus dulcis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lacto-N-difucohexaitol + H2O | poor substrate | Prunus dulcis | ? | - |
? | |
| lacto-N-fucopentaitol + H2O | lacto-N-fucopentaitol II or III, no substrate: lacto-N-fucopentaitol I | Prunus dulcis | ? | - |
? | |
| lacto-N-fucopentaitol II + H2O | - |
Prunus dulcis | ? | - |
? | |
| lacto-N-fucopentaitol III + H2O | - |
Prunus dulcis | ? | - |
? | |
| additional information | no hydrolysis of fucose alpha-1,6 or alpha-1,2-linkages to N-acetylglucosamine, as in IgG glycopeptides or 2'-fucosyllactitol | Prunus dulcis | ? | - |
? | |
| additional information | the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine | Prunus dulcis | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Prunus dulcis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.8 | about half-maximal activity at pH 4 and 6.8 | Prunus dulcis |
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