Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | 106.16% activity at 1 mM | Penicillium cyclopium | |
Tris | 103.79% activity at 1 mM | Penicillium cyclopium | |
Urea | 112.79% activity at 1 mM | Penicillium cyclopium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 40.73% residual activity at 10 mM | Penicillium cyclopium | |
Cr2+ | 48.25% residual activity at 10 mM | Penicillium cyclopium | |
Cu2+ | 16.41% residual activity at 10 mM | Penicillium cyclopium | |
EDTA | 80.58% residual activity at 5 mM | Penicillium cyclopium | |
Fe3+ | 64.61% residual activity at 5 mM | Penicillium cyclopium | |
Ni2+ | 67.71% residual activity at 10 mM | Penicillium cyclopium | |
Pb2+ | 20.39% residual activity at 10 mM | Penicillium cyclopium | |
SDS | 34.15% residual activity at 10 mM | Penicillium cyclopium | |
Zn2+ | 16.85% residual activity at 10 mM | Penicillium cyclopium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.45 | - |
dextran T2000 | at pH 5.0 and 50°C | Penicillium cyclopium | |
2.61 | - |
dextran T70 | at pH 5.0 and 50°C | Penicillium cyclopium | |
2.72 | - |
dextran T5 | at pH 5.0 and 50°C | Penicillium cyclopium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 109.95% activity at 10 mM | Penicillium cyclopium | |
Li+ | 116.28% activity at 1 mM | Penicillium cyclopium | |
Na+ | 111.48% activity at 5 mM | Penicillium cyclopium | |
NH4+ | 107.58% activity at 1 mM | Penicillium cyclopium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | Penicillium cyclopium | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? | |
dextran + H2O | Penicillium cyclopium CICC-4022 | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium cyclopium | - |
- |
- |
Penicillium cyclopium CICC-4022 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sepharose column chromatography | Penicillium cyclopium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? | |
dextran + H2O | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | Penicillium cyclopium CICC-4022 | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? | |
dextran T20 + H2O | 90.64% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T20 + H2O | 90.64% activity compared to dextran T70 | Penicillium cyclopium CICC-4022 | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T2000 + H2O | 97% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T2000 + H2O | 97% activity compared to dextran T70 | Penicillium cyclopium CICC-4022 | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T3 + H2O | 65.17% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T40 + H2O | 98.5% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T5 + H2O | 65.54% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T500 + H2O | 99.62% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
dextran T70 + H2O | 100% activity | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
additional information | the enzyme has no effects on cellulose, sucrose, chitin, chitosan and beta-cyclodextrin | Penicillium cyclopium | ? | - |
? | |
additional information | the enzyme has no effects on cellulose, sucrose, chitin, chitosan and beta-cyclodextrin | Penicillium cyclopium CICC-4022 | ? | - |
? | |
soluble starch + H2O | 20.83% activity compared to dextran T70 | Penicillium cyclopium | ? | - |
? | |
soluble starch + H2O | 20.83% activity compared to dextran T70 | Penicillium cyclopium CICC-4022 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Penicillium cyclopium |
Synonyms | Comment | Organism |
---|---|---|
1,6-alpha-D-glucan-6-glucanohydrolase | - |
Penicillium cyclopium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Penicillium cyclopium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | when temperature varying from 35 to 60°C, the relative activity is always higher than 54.55% | Penicillium cyclopium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | after 60 min 45°C and 50°C, the dextranase activity remains stable with the values being 98.09% and 62.21% of relative enzymatic activity, respectively | Penicillium cyclopium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
208.45 | - |
dextran T5 | at pH 5.0 and 50°C | Penicillium cyclopium | |
211.97 | - |
dextran T70 | at pH 5.0 and 50°C | Penicillium cyclopium | |
223.53 | - |
dextran T2000 | at pH 5.0 and 50°C | Penicillium cyclopium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Penicillium cyclopium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | with pH ranged from 3.0 to 6.0, the relative enzyme activity is higher than 72.67% | Penicillium cyclopium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 7 | the relative enzymatic activity of dextranase stored at pH 2.5-7.0 for 1 h and 24 h, is 89.22% and 84.58%, respectively | Penicillium cyclopium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
76.636 | - |
dextran T5 | at pH 5.0 and 50°C | Penicillium cyclopium | |
81.215 | - |
dextran T70 | at pH 5.0 and 50°C | Penicillium cyclopium | |
91.237 | - |
dextran T2000 | at pH 5.0 and 50°C | Penicillium cyclopium |