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Literature summary for 3.2.1.11 extracted from

  • Park, T.S.; Jeong, H.J.; Ko, J.A.; Ryu, Y.B.; Park, S.J.; Kim, D.; Kim, Y.M.; Lee, W.S.
    Biochemical characterization of thermophilic dextranase from a thermophilic bacterium, Thermoanaerobacter pseudethanolicus (2012), J. Microbiol. Biotechnol., 22, 637-641.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
dextran T500 Km value is 2.5 mg/ml, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
additional information
-
dextran 742CB Km value is 2.9 mg/ml, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
additional information
-
dextran T2000 Km value is 6.4 mg/ml, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Thermoanaerobacter pseudethanolicus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter pseudethanolicus
-
-
-
Thermoanaerobacter pseudethanolicus ATCC 33223 39E
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoanaerobacter pseudethanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
substrate alternan, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
2.7
-
substrate dextran 742CB, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
6.2
-
substrate dextran T500, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
7.37
-
substrate dextran T2000, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alternan + H2O
-
Thermoanaerobacter pseudethanolicus ?
-
?
alternan + H2O
-
Thermoanaerobacter pseudethanolicus ATCC 33223 39E ?
-
?
dextran 742CB + H2O
-
Thermoanaerobacter pseudethanolicus ?
-
?
dextran 742CB + H2O
-
Thermoanaerobacter pseudethanolicus ATCC 33223 39E ?
-
?
dextran T2000 + H2O
-
Thermoanaerobacter pseudethanolicus ?
-
?
dextran T2000 + H2O
-
Thermoanaerobacter pseudethanolicus ATCC 33223 39E ?
-
?
dextran T500 + H2O
-
Thermoanaerobacter pseudethanolicus ?
-
?
dextran T500 + H2O
-
Thermoanaerobacter pseudethanolicus ATCC 33223 39E ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Thermoanaerobacter pseudethanolicus

Synonyms

Synonyms Comment Organism
TPDex
-
Thermoanaerobacter pseudethanolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermoanaerobacter pseudethanolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
dextran 742CB pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
8.1
-
dextran T500 pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
10.2
-
dextran T2000 pH 5.2, 70°C Thermoanaerobacter pseudethanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
Thermoanaerobacter pseudethanolicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.1 8.5 70°C, stable for 7.4 h Thermoanaerobacter pseudethanolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
dextran T2000 kcat_/Km value is 5.9 ml per mg and s, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
additional information
-
dextran 742CB Km value is 1.25ml per mg and s, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus
additional information
-
dextran T500 Km value is 3.3 ml per mg and s, pH 5.2, 70°C Thermoanaerobacter pseudethanolicus