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Literature summary for 3.2.1.11 extracted from

  • Gan, W.; Zhang, H.; Zhang, Y.; Hu, X.
    Biosynthesis of oligodextrans with different Mw by synergistic catalysis of dextransucrase and dextranase (2014), Carbohydr. Polym., 112, 387-395.
    View publication on PubMed

Application

Application Comment Organism
synthesis investigation on various adding times of dextranase to the dextransucrase system to reveal the synergistic processes of dextransucrase and dextranase. Dextranase added into the dextransucrase-sucrose system at different times gives rise to different main dextran products. Dextranase added into sucrose system at the same time with dextransucrase synthesizes low molecular weight dextran targeted to 5 kDa, while dextranase added during the reaction process of dextransucrase directionally prepares dextran with medium Mw of 10 kDa and 20 kDa. The synthesized oligodextrans are mainly composed of alpha-1,6-glycosidic linkages and low alpha-1,3-glycosidic branches Polystichum aculeatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
Dextran substrate dextran 70 kDa, pH 5.0, 35°C Polystichum aculeatum
0.0005
-
Dextran substrate dextran 40 kDa, pH 5.0, 35°C Polystichum aculeatum
0.00175
-
Dextran substrate dextran 20 kDa, pH 5.0, 35°C Polystichum aculeatum

Organism

Organism UniProt Comment Textmining
Polystichum aculeatum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Polystichum aculeatum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
Polystichum aculeatum ?
-
?

Synonyms

Synonyms Comment Organism
dextransucrase
-
Polystichum aculeatum