Literature summary for 3.2.1.11 extracted from
Ryu, H.; Jin, X.; Lee, J.; Woo, H.; Kim, Y.; Kim, G.; Seo, E.; Kang, H.; Kim, J.; Cho, D.; Kimura, A.; Kim, D.
Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities (2010), Enzyme Microb. Technol., 47, 212-215.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene dex2, expression of the engineered fusion enzyme DSXR in Escherichia coli strain BL21(DE3)pLysS, optimization of protein expression conditions for enhancement of protein production by the effects of three-level-three-factors and their mutual interaction in Escherichia coli |
Pseudarthrobacter oxydans |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
generation of an engineered fusion enzyme of dextransucrase, from dsrBCB4 gene from Leuconostoc mesenteroides B-1299CB4, and Arthrobacter oxydans dextranase, i.e. DSXR. DSXR is potentially useful for reliable production of long isomalto-oligosaccharides from sucrose by a one-step reaction |
Pseudarthrobacter oxydans |
Organism
Organism |
UniProt |
Comment |
Textmining |
Pseudarthrobacter oxydans |
A9UKG4 |
gene dex2 |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
additional information |
- |
activities of recombinant fusion protein and of wild-type dextranase in fermentation, overview |
Pseudarthrobacter oxydans |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
5.2 |
- |
recombinant wild-type dextranase |
Pseudarthrobacter oxydans |
5.8 |
- |
recombinant engineered fusion protein DSXR |
Pseudarthrobacter oxydans |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
5 |
6.4 |
great loss in activity below and above that values, recombinant wild-type dextranase and engineered fusion protein DSXR |
Pseudarthrobacter oxydans |