Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) CodonPlus-RIL | Streptococcus mutans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of five truncated SmDexs by deleting the N-terminal variable region, the glucan-binding site, or the C-terminal variable region. Two truncation-mutant enzymes devoid of C-terminal variable region or of C-terminal variable region and N-terminal variable region are catalytically active, thereby indicating that the two regions are not essential for the catalytic activity, mutant structures compared to the wild-type enzyme, overview | Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89800 | - |
x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE | Streptococcus mutans |
94537 | - |
x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE | Streptococcus mutans |
95400 | - |
x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | F5BA50 | - |
- |
Streptococcus mutans ATCC 25175 | F5BA50 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) CodonPlus-RIL by nickel affinity chromatography, ultrafilatration, and hydrophobic interaction and anion exchange chromatography | Streptococcus mutans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cells are cultivated in brain-heart infusion medium | Streptococcus mutans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.02 | - |
purified recombinant enzyme SmDex95, the 95 kDa variant, pH 5.0, temperature not specified in the publication | Streptococcus mutans |
8.21 | - |
purified recombinant enzyme SmDex90, the 90 kDa variant, pH 5.0, temperature not specified in the publication | Streptococcus mutans |
Storage Stability | Organism |
---|---|
4°C, 2 mM NaN3, 6 months, the purified SmDex90 is proteolytically degraded to more than seven polypeptides of 23-70 kDa during long storage | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | - |
Streptococcus mutans | ? | - |
? | |
dextran + H2O | - |
Streptococcus mutans ATCC 25175 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE | Streptococcus mutans |
More | the enzyme comprises four regions from the N- to C-termini: N-terminal variable region, N-VR, conserved region, CR, glucan-binding site, GBS, and C-terminal variable region, C-VR | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
endo-dextranase | - |
Streptococcus mutans |
More | the enzyme belongs to the glycoside hydrolase family (GH) 66 | Streptococcus mutans |
SmDex | - |
Streptococcus mutans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzymes, stable up to | Streptococcus mutans |
General Information | Comment | Organism |
---|---|---|
additional information | N-terminal variable region and C-terminal variable region of the enzyme are not essentially required for catalytic activity, but induce hindered substrate binding to the active site | Streptococcus mutans |