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Literature summary for 3.2.1.11 extracted from
- Construction of a fusion enzyme of dextransucrase and dextranase: application for one-step synthesis of isomalto-oligosaccharides (2009), Enzyme Microb. Technol., 44, 159-164.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the dextransucrase-dextranase fusion protein is expressed in Escherichia coli BL21(DE3) cells | Pseudarthrobacter oxydans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | strong inhibitor | Pseudarthrobacter oxydans |  |
| Hg2+ | strong inhibitor | Pseudarthrobacter oxydans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | stimulates activity of the dextransucrase-dextranase fusion protein | Pseudarthrobacter oxydans | |
| K+ | stimulates activity of the dextransucrase-dextranase fusion protein | Pseudarthrobacter oxydans | |
| Li+ | stimulates activity of the dextransucrase-dextranase fusion protein | Pseudarthrobacter oxydans | |
| Ni2+ | stimulates activity of the dextransucrase-dextranase fusion protein | Pseudarthrobacter oxydans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | - |
dextransucrase-dextranase fusion protein, SDS-PAGE | Pseudarthrobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | Pseudarthrobacter oxydans | - |
oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudarthrobacter oxydans | A9UKG4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the dextransucrase-dextranase fusion protein is partially purified by DEAE-Sepharose column chromatography | Pseudarthrobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | - |
Pseudarthrobacter oxydans | oligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dex2 | - |
Pseudarthrobacter oxydans |
| DXSR | fusion protein of endo-dextranase and dextransucrase | Pseudarthrobacter oxydans |
| endo-dextranase | - |
Pseudarthrobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Pseudarthrobacter oxydans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
stable at temperatures below 28°C | Pseudarthrobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudarthrobacter oxydans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.4 | - |
Pseudarthrobacter oxydans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | more than 80% activity at pH 6.0-8.0 | Pseudarthrobacter oxydans |
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Release 2023.1 (January 2023)
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