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Literature summary for 3.2.1.11 extracted from
- Purification and properties of extracellular dextranase from a Bacillus sp (2003), J. Chromatogr. B, 796, 315-326.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paenibacillus illinoisensis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
x * 76000, SDS-PAGE, isoenzyme 3 | Paenibacillus illinoisensis |
| 89000 | - |
x * 89000, SDS-PAGE, isoenzyme 2 | Paenibacillus illinoisensis |
| 110000 | - |
x * 110000, SDS-PAGE, isoenzyme 1 | Paenibacillus illinoisensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | Paenibacillus illinoisensis | - |
fragments of dextran | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus illinoisensis | - |
isoenzyme 1 | - |
| Paenibacillus illinoisensis | - |
isoenzyme 2 | - |
| Paenibacillus illinoisensis | - |
isoenzyme 3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, 20% polyethylene glycol 6000, interphase adsorption, DEAE-Sepharose | Paenibacillus illinoisensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
release of 78.5 mg of Remazol Blue R-250 dye/min at 50°C | Paenibacillus illinoisensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | - |
Paenibacillus illinoisensis | fragments of dextran | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, SDS-PAGE, isoenzyme 1 | Paenibacillus illinoisensis |
| ? | x * 76000, SDS-PAGE, isoenzyme 3 | Paenibacillus illinoisensis |
| ? | x * 89000, SDS-PAGE, isoenzyme 2 | Paenibacillus illinoisensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| extracellular dextranase | - |
Paenibacillus illinoisensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Paenibacillus illinoisensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | approx. 35% of maximal activity at 30°C, approx. 10% of maximal activity at 60°C | Paenibacillus illinoisensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
no loss of activity after 60 min | Paenibacillus illinoisensis |
| 60 | - |
approx. 50% loss of activity | Paenibacillus illinoisensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.8 | - |
Paenibacillus illinoisensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | approx. 50% of maximal activity at pH 5.2 and pH 8.0 | Paenibacillus illinoisensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Paenibacillus illinoisensis | isoelectric focusing, isoenzyme 1 | - |
4 |
| Paenibacillus illinoisensis | isoelectric focusing, isoenzyme 2 | - |
4.2 |
| Paenibacillus illinoisensis | isoelectric focusing, isoenzyme 3 | - |
5 |
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