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Literature summary for 3.2.1.109 extracted from
- An endogalactosaminidase from Streptomyces griseus (1975), Can. J. Biochem., 53, 1237-1249.
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing, no loss of activity | Streptomyces griseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Streptomyces griseus |  |
| EDTA | - |
Streptomyces griseus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces griseus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalactosaminoglycan + H2O | Streptomyces griseus | high molecular weight substrate obtained from Neurospora | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces griseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Streptomyces griseus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 8 months, distilled water, stable to repeated freezing and thawing | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | sporeling detaching activity, not: N-acetyl-oligogalactosaminoglycan and chitosan | Streptomyces griseus | ? | - |
? | |
| oligogalactosaminoglycan + H2O | endohydrolysis of 1,4-alpha-galactosaminidic linkages | Streptomyces griseus | ? | - |
? | |
| polygalactosaminoglycan + H2O | high molecular weight substrate obtained from Neurospora | Streptomyces griseus | galactosamine + GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN | - |
? | |
| polygalactosaminoglycan + H2O | high molecular weight substrate obtained from Neurospora | Streptomyces griseus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Streptomyces griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat stability increases at less acid pH | Streptomyces griseus |
| 20 | - |
pH 2, inactivated within a few min | Streptomyces griseus |
| 100 | - |
half-life: 3 min | Streptomyces griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
sporeling-detaching activity | Streptomyces griseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | very low activity at pH 3 and 8 | Streptomyces griseus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
20°C, inactivated within a few min | Streptomyces griseus |
| 7 | - |
quite stable at neutrality | Streptomyces griseus |
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